CHROMOSOMAL LOCATION OF FERTILITY RESTORING GENES FOR WILD ABORTIVE CYTOPLASMIC MALE-STERILITY USING PRIMARY TRISOMICS IN RICE

被引：106

作者：

BHARAJ, TS

VIRMANI, SS

KHUSH, GS

机构：

[1] International Rice Research Institute, Manila

来源：

EUPHYTICA | 1995年 / 83卷 / 03期

关键词：

RICE; ORYZA SATIVA; CYTOPLASMIC MALE STERILITY; FERTILITY RESTORATION; CHROMOSOMAL GENE LOCATION; PRIMARY TRISOMICS;

D O I：

10.1007/BF01678126

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 [作物学];

摘要：

Identification and location of fertility restoring genes facilitates their deployment in a hybrid breeding program involving cytoplasmic male sterility (CMS) system. The study aimed to locate fertility restorer genes of CMS-WA system on specific chromosomes of rice using primary trisomics of IR36 (restorer), CMS (IR58025A) and maintainer (IR58025B) lines. Primary trisomic series (Triple 1 to 12) was crossed as maternal parent with the maintainer line IR58025B. The selected trisomic and disomic F-1 plants were testcrossed as male parents with the CMS line IR58025A. Plants in testcross families derived from disomic F-1 plants (Group I crosses) were all diploid; however, in the testcross families derived from trisomic F-1 plants (Group II crosses), some trisomic plants were observed. Diploid plants in all testcross families were analyzed for pollen fertility using 1% IKI stain. All testcross families from Group I crosses segregated in the ratio of 2 fertile: 1 partially fertile + partially sterile : 1 sterile plants indicating that fertility restoration was controlled by two independent dominant genes; one of the genes was stronger than the other. Testcross families from Group II crosses segregated in 2 fertile : 1 partially fertile + partially sterile : 1 sterile plants in crosses involving Triple 1, 4, 5, 6, 8, 9, 11 and 12, but families involving triple 7 and triple 10 showed significantly higher X(2) values, indicating that the two fertility restorer genes were located on chromosome 7 and 10. Stronger restorer gene (Rf-WA-1) was located on chromosome 7 and weaker restorer gene (Rf-WA-2) was located on chromosome 10. These findings should facilitate tagging of these genes with molecular markers with the ultimate aim to practice marker-aided selection for fertility restoration ability.

引用

页码：169 / 173

页数：5

共 17 条

[1]

Bahl P.N., Maan S.S., Chromosomal location of male fertility restorer genes in six lines of common wheat, Crop Science, 13, pp. 317-320, (1973)

[2]

Bharai T.S., Bains S.S., Sidhu G.S., Gagneja M.R., Genetics of fertility restoration of ‘wild abortive’ cytoplasmic male sterility in rice, Oryza sativa L, Euphytica, 56, pp. 199-203, (1991)

[3]

Govinda Raj K., Virmani S.S., Genetics of fertility restoration of ‘WA’ type cytoplasmic male sterility in rice, Crop Sci., 28, pp. 787-792, (1988)

[4]

Khush G.S., Singh R.J., Sur A.C., Librojo A.L., Primary trisomics of rice: Origin, morphology, cytology and use in linkage mapping, Genetics, 107, pp. 141-163, (1984)

[5]

Li Y.C., Yuan L.P., Genetic analysis of fertility restoration in male sterile lines of rice, Rice Genetics, pp. 617-632, (1986)

[6]

Lin S.C., Yuan L.P., Hybrid rice breeding in China, Innovative approaches to rice breeding, pp. 35-51, (1980)

[7]

Shinjyo C., Cytoplasmic-genetic male sterility in cultivated rice, Oryza sativa L. II. The inheritance of male sterility, The Japanese journal of genetics, 44, pp. 149-156, (1969)

[8]

Shinjyo C., Genetical studies of cytoplasmic male sterility and fertility restoration in rice, Oryza sativa L, Sci. Bull. Coll Agr Univ. Ryukyus, 22, pp. 1-57, (1975)

[9]

Shinjyo C., Nishime R., Watanabe Y., Inheritance of fertility restoring gene Rfx and Rf in male sterile cytoplasm derived from vanety Lead rice, Japan J. Breed, pp. 130-131, (1974)

[10]

Shinyjo C., Watanabe Y., Allelism test of 2 genes showing fertility restoring effect for male sterile cytoplasm of Lead rice and their inheritance, Japan J. Breed, pp. 70-71, (1977)

← 1 2 →