NORMAL FERTILIZATION AND EMBRYO DEVELOPMENT BY INTRACYTOPLASMIC SPERM INJECTION OF ROUND-HEADED ACROSOMELESS SPERM

被引：44

作者：

BOURNE, H ^{[1
]}

LIU, DY ^{[1
]}

CLARKE, GN ^{[1
]}

BAKER, HWG ^{[1
]}

机构：

[1] UNIV MELBOURNE,CARLTON,VIC,AUSTRALIA

来源：

FERTILITY AND STERILITY | 1995年 / 63卷 / 06期

关键词：

ROUND HEADED ACROSOMELESS SPERM; INTRACYTOPLASMIC SPERM INJECTION; MALE INFERTILITY;

D O I：

10.1016/S0015-0282(16)57620-1

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: To investigate the ability of round-headed acrosomeless sperm to bind to the human zona pellucida (ZP) and oolemma and to fertilize human oocytes by intracytoplasmic sperm injection. Design: Oocytes that had failed to fertilize in IVF were used for sperm-ZP and spermoolemma binding tests. Sperm from a fertile donor was used as a control for oocyte variability. Intracytoplasmic sperm injection was used for assisted fertilization. Setting: University- and hospital-based reproductive research laboratory and tertiary referral IVF program. Patients: Case study of a couple in which the man has 100% round-headed acrosomeless sperm in the ejaculate. Main Outcome Measure: Fertilization and embryo development and the ability of sperm to bind to the ZP and oolemma. Results: No ZP or oolemma binding was achieved, but normal fertilization and embryo development was obtained after intracytoplasmic injection of round-headed acrosomeless sperm. However, no pregnancy was achieved after the transfer of two cleaving embryos. Conclusions: Normal fertilization and embryo development from round-headed acrosomeless sperm is possible with intracytoplasmic sperm injection. However, it remains to be reported whether pregnancy can result from fertilization with this type of sperm defect.

引用

页码：1329 / 1332

页数：4

共 5 条

[1] Schirren C.G., Holstein A.F., Schirren C., Uber die morphogenese rundkopfiger spermatozoen des menschen, Andrologia, 3, pp. 117-125, (1971)
[2] von Bernhardi R., de Ioannes A.E., Blanco L.P., Herrera E., Bustos-Obregon E., Vigil P., Round-headed spermatozoa: a model to study the role of the acrosome in early events of gamete interaction, Andrologia, 22, pp. 12-20, (1990)
[3] Lalonde L., Langlais J., Antaki P., Chapdelaine A., Roberts K.D., Bleau G., Male infertility associated with round-headed acrosomeless spermatozoa, Fertil Steril, 49, pp. 316-321, (1988)
[4] Lanzendorf S., Maloney M., Ackerman S., Acosta A., Hodgen G., Fertilizing potential of acrosome defective sperm following microsurgical injection into eggs, Gamete Res, 19, pp. 329-337, (1988)
[5] Blanchard Y., Lescoat D., Le Lannou D., Anomalous distribution of nuclear basic proteins in round-headed human spermatozoa, Andrologia, 22, pp. 549-555, (1990)

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