PARAQUAT TOXICITY AND PYRIDINE-NUCLEOTIDE COENZYME SYNTHESIS - A DATA CORRECTION

被引：2

作者：

BROWN, OR

SEITHER, RL

机构：

[1] Department of Microbiology, the School of Medicine

[2] Department of Veterinary Biomedical Sciences, the College of Veterinary Medicine, University of Missouri, Columbia

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 1990年 / 8卷 / 02期

关键词：

Free radicals; Nicotinatemononucleotide pyrophosphorylase (carboxylating); Oxidant stress; Oxygen toxicity; Paraquat toxicity; Pyridine nucleotide coenzymes; Superoxide;

D O I：

10.1016/0891-5849(90)90082-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The decrease in pyridine nucleotide coenzymes which occurs during poisoning of Escherichia coli by hyperbaric oxygen or paraquat is not due to impairment of nicotinatemononucleotide pyrophosphorylase (carboxylating) [EC 2.4.2.19] as was previously proposed (Brown, O.R. et al. Biochem. Biophys. Res. Commun. 91:982-990; 1979). This was shown directly using extracts of E. coli, prepared after exposure to 1 mM paraquat or 4.2 atmospheres of oxygen. The enzyme also was not impaired in Neurospora crassa by 1 mM paraquat. A naturally-occurring, non-dialyzable inhibitor of the enzyme was found in E. coli extracts. The inhibitor caused the erroneous, low nicotinatemononucleotide pyrophosphorylase (carboxylating) activities previously reported in extracts of E. coli poisoned by paraquat. © 1990.

引用

页码：113 / 116

页数：4

共 12 条

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[2] Brown, Specific enzymatic sites and cellular mechanisms of oxygen toxicity, Biochemical and clinical aspects of oxygen, pp. 755-766, (1979)
[3] Brown, Yein, Boehm, Foundin, Song, Oxygen poisoning of NAD biosynthesis: a proposed site of cellular oxygen toxicity, Biochem. Biophys. Res. Commun., 91, pp. 982-990, (1979)
[4] Brown, Song, Pyridine nucleotide coenzyme biosynthesis: a cellular site of oxygen toxicity, Biochem. Biophys. Res. Commun., 93, pp. 172-178, (1980)
[5] Heitkamp, Brown, Inhibition of NAD biosynthesis by paraquat in Escherichia coli, Biochimica et Biophysica Acta, 676, pp. 345-349, (1981)
[6] Seither, Brown, Toxicity of paraquat and nitrofurantoin via induction of genetic stringency, J. Tox. Environm. Health, 14, pp. 763-771, (1984)
[7] Brown, Yein, Dihydroxyacid dehydratase
[8] the site of hyperbaric oxygen poisoning in branched-chain amino acid biosynthesis, Biochem. Biophys. Res. Commun., 85, pp. 1219-1224, (1978)
[9] Davis, DeSerres, Genetic and microbiological research techniques for Neurospora crassa, Methods in enzymology, 17 A, pp. 79-149, (1970)
[10] Gholson, Ueda, Ogasawara, Henderson, The enzymatic conversion of guinolinate to nicotinic acid mononucleotide in mammalian liver, J. Biol. Chem., 239, pp. 1208-1214, (1964)

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