COMPARISON OF A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC AND AN ENZYME IMMUNOASSAY TECHNIQUE FOR QUANTITATION OF THEOPHYLLINE IN SERUM

被引：14

作者：

BOECKX, RL ^{[1
]}

FRITH, EM ^{[1
]}

SIMONS, FER ^{[1
]}

机构：

[1] HLTH SCI CTR,DEPT PEDIAT,685 BANNATYNE AVE,WINNIPEG R3E 0W1,MANITOBA,CANADA

来源：

THERAPEUTIC DRUG MONITORING | 1979年 / 1卷 / 01期

关键词：

EMIT®; method; HPLC assay; Theophylline;

D O I：

10.1097/00007691-197901000-00007

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

High performance liquid chromatographic (HPLC) and enzyme immunoassay (EMIT®) methods for quantitating theophylline concentrations in serum were evaluated. For both methods, standard curves were linear over the therapeutic range of serum concentrations. Precision was acceptable, with coefficients of variation being less than 9%. The HPLC assay was slightly, but not significantly, more precise. No interference was noted in either method by caffeine, theobromine, diphylline, 8-chlorotheophylline, or by metabolites such as 1-methylxanthine, and 3-methylxanthine, or by potentially interfering compounds such as urea and uric acid. The two methods correlated well, with a correlation coefficient of 0.98%. The enzyme immunoassay was superior in terms of lower costs, greater ease of performance, and potential for automation. © 1979 Raven Press, New York.

引用

页码：65 / 73

页数：9

共 15 条

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