ELECTROPORATION OF PKK1 SILVER-RESISTANCE PLASMID FROM PSEUDOMONAS-STUTZERI AG259 INTO PSEUDOMONAS-PUTIDA CYM318

被引：15

作者：

TREVORS, JT

STARODUB, ME

机构：

[1] Department of Environmental Biology, Rm. 316, Chemistry-Microbiology, University of Guelph, Guelph, N1G 2W1, Ontario

来源：

CURRENT MICROBIOLOGY | 1990年 / 21卷 / 02期

关键词：

D O I：

10.1007/BF02091827

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Plasmid pKK1 (49.4 Mda), which encodes for Ag+ resistance, was isolated from Pseudomonas stutzeri AG259 (pKK1) and introduced into Pseudomonas putida CYM318 by high-voltage electroporation. Upon acquiring pKK1, P. putida CYM318 became resistant to AgNO3. This demonstrated that electroporation is a useful method to introduce a nonconjugative metal-resistance plasmid into a bacterial strain and to conduct further comparative research on Ag accumulation. © 1990 Springer-Verlag New York Inc.

引用

页码：103 / 107

页数：5

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