THE MECHANISM OF ACTION OF PEPPERMINT OIL ON GASTROINTESTINAL SMOOTH-MUSCLE - AN ANALYSIS USING PATCH CLAMP ELECTROPHYSIOLOGY AND ISOLATED TISSUE PHARMACOLOGY IN RABBIT AND GUINEA-PIG

被引：181

作者：

HILLS, JM ^{[1
]}

AARONSON, PI ^{[1
]}

机构：

[1] UNITED MED & DENT SCH GUYS & ST THOMAS HOSP,ST THOMAS HOSP,DEPT PHARMACOL,LONDON,ENGLAND

来源：

GASTROENTEROLOGY | 1991年 / 101卷 / 01期

关键词：

D O I：

10.1016/0016-5085(91)90459-X

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

An investigation of the mechanism of peppermint oil action was performed using isolated pharmacological preparations from guinea pig large intestine and patch clamp electrophysiology techniques on rabbit jejunum. Peppermint oil relaxed carbachol-contracted guinea pig taenia coli (IC50, 22.1 μg/mL) and inhibited spontaneous activity in the guinea pig colon (IC50, 25.9 μg/mL) and rabbit jejunum (IC50, 15.2 μg/mL). Peppermint oil markedly attenuated contractile responses in the guinea pig taenia coli to acetylcholine, histamine, 5-hydroxytryptamine, and substance P. Peppermint oil reduced contractions evoked by potassium depolarization and calcium contractions evoked in depolarizing Krebs solutions in taenia coli. Potential-dependent calcium currents recorded using the whole cell clamp configuration in rabbit jejunum smooth muscle cells were inhibited by peppermint oil in a concentration-dependent manner. Peppermint oil both reduced peak current amplitude and increased the rate of current decay. The effect of peppermint oil resembled that of the dihydropyridine calcium antagonists. It is concluded that peppermint oil relaxes gastrointestinal smooth muscle by reducing calcium influx. © 1991.

引用

页码：55 / 65

页数：11

共 38 条

[1] CALCIUM CURRENTS IN SINGLE ISOLATED SMOOTH-MUSCLE CELLS FROM THE RABBIT EAR ARTERY IN NORMAL-CALCIUM AND HIGH-BARIUM SOLUTIONS [J].

AARONSON, PI ;

BOLTON, TB ;

LANG, RJ ;

MACKENZIE, I .

JOURNAL OF PHYSIOLOGY-LONDON, 1988, 405 :57-75

[2]

ARUNLAKSHANA O, 1959, BR J PHARM CHEMOTHER, V14, P45

[3] NITRENDIPINE BLOCK OF CARDIAC CALCIUM CHANNELS - HIGH-AFFINITY BINDING TO THE INACTIVATED STATE [J].

BEAN, BP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (20) :6388-6392

[4]

BEAN BP, 1986, CIRC RES, V59, P220

[5] COMPARISON OF THE EXCITATORY ACTIONS OF SUBSTANCE-P, CARBACHOL, HISTAMINE AND PROSTAGLANDIN-F2-ALPHA ON THE SMOOTH-MUSCLE OF THE TAENIA OF THE GUINEA-PIG CECUM [J].

BENHAM, CD ;

BOLTON, TB .

BRITISH JOURNAL OF PHARMACOLOGY, 1983, 80 (03) :409-420

[6] SPONTANEOUS TRANSIENT OUTWARD CURRENTS IN SINGLE VISCERAL AND VASCULAR SMOOTH-MUSCLE CELLS OF THE RABBIT [J].

BENHAM, CD ;

BOLTON, TB .

JOURNAL OF PHYSIOLOGY-LONDON, 1986, 381 :385-406

[7]

BERTACCINI G, 1982, MEDIATORS DRUGS GAST, P201

[8] MECHANISMS OF ACTION OF TRANSMITTERS AND OTHER SUBSTANCES ON SMOOTH-MUSCLE [J].

BOLTON, TB .

PHYSIOLOGICAL REVIEWS, 1979, 59 (03) :606-718

[9]

BOLTON TB, 1988, ANN NY ACAD SCI, V522, P32

[10]

BORNSTEIN JC, 1984, NEUROSCIENCE, V11, P723, DOI 10.1016/0306-4522(84)90055-1

← 1 2 3 4 →