IMMUNOREACTIVITIES FOR ANTISERA TO 3 PUTATIVE NEUROPEPTIDES OF THE RAT MELANIN-CONCENTRATING HORMONE PRECURSOR ARE COEXPRESSED IN NEURONS OF THE RAT LATERAL DORSAL HYPOTHALAMUS

被引：53

作者：

RISOLD, PY

FELLMANN, D

RIVIER, J

VALE, W

BUGNON, C

机构：

[1] FAC MED BESANCON, HISTOL EMBRYOL CYTOGENET LAB,CNRS,URA 0561, PL ST JACQUES, F-25030 BESANCON, FRANCE

[2] SALK INST BIOL STUDIES, CLAYTON FDN LABS PEPTIDES BIOL, SAN DIEGO, CA 92138 USA

来源：

NEUROSCIENCE LETTERS | 1992年 / 136卷 / 02期

关键词：

ANTISERUM; NEUROPEPTIDE GLYCINE-GLUTAMIC ACID; NEUROPEPTIDE GLUTAMIC ACID-ISOLEUCINEAMIDE; RAT MELANIN-CONCENTRATING HORMONE; RAT BRAIN;

D O I：

10.1016/0304-3940(92)90035-6

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Antisera (AS) raised against rat melanin-concentrating hormone (rMCH) and against two additional peptides sequences derived from the rat MCH precursor (neuropeptide glutamic acid-isoleucineamide (NEI), and neuropeptide glycine-glutamic acid (NGE)) exclusively stained the hypothalamic neurons previously described using AS to salmon MCH, human somatocrinin 1-37 (GRF37) and alpha-MSH. Liquid phase and dot-blot controls for specificity indicated that rMCH-, NEI- and NGE-AS bound epitopes recognized by sMCH-, alpha-MSH- and GRF-37-AS, respectively. The distinct intracellular patterns of immunoreactivity obtained in control animals with rMCH-, NGE- and NEI-AS, as well as the changes observed after intracerebroventricular injection of colchicine matched previous findings using sMCH-, GRF37- and alpha-MSH-AS.

引用

页码：145 / 149

页数：5

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