A FLUOROGENIC ACTINOMETER FOR NUCLEIC-ACID PHOTOCHEMISTRY UTILIZING 1-DEAZAPURINE N(3)-OXIDES

被引：5

作者：

BLANEY, R ^{[1
]}

ALNAKIB, T ^{[1
]}

DAVIES, RJH ^{[1
]}

机构：

[1] QUEENS UNIV BELFAST,SCH BIOL & BIOCHEM,BELFAST BT9 7BL,ANTRIM,NORTH IRELAND

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1993年 / 57卷 / 02期

关键词：

D O I：

10.1111/j.1751-1097.1993.tb02305.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A convenient and very sensitive actinometry system for UV radiation with wavelengths in the range 250-310 nm is described, which is based on the fluorogenic photorearrangement of 1-deazapurine N(3)-oxide. The product, 1-deazapur-in-2-one, can be estimated fluorometrically in the presence of the N-oxide. Quantum yields measured between 250 and 310 nm for the N(3)-oxides of 1-deazapurine and 1-methyl-1-deazapurine fall in the range 0.13-0.15 and do not vary significantly with wavelength. There is potential for using 1-deazapurine N(3)-oxides as UV-photoactivable fluorophores.

引用

页码：380 / 382

页数：3

共 16 条

[1] THE PHOTOCHEMISTRY OF D(T-A) IN AQUEOUS-SOLUTION AND IN ICE
BOSE, SN
KUMAR, S
DAVIES, RJH
SETHI, SK
MCCLOSKEY, JA
[J]. NUCLEIC ACIDS RESEARCH, 1984, 12 (20) : 7929 - 7947
[2] FORMATION OF AN ADENINE-THYMINE PHOTOADDUCT IN THE DEOXYDINUCLEOSIDE MONOPHOSPHATE D(TPA) AND IN DNA
BOSE, SN
DAVIES, RJH
SETHI, SK
MCCLOSKEY, JA
[J]. SCIENCE, 1983, 220 (4598) : 723 - 725
[3] A NEW REUSABLE CHEMICAL ACTINOMETER FOR UV IRRADIATION IN THE 248-334 NM RANGE
BRAUER, HD
SCHMIDT, R
[J]. PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1983, 37 (05) : 587 - 591
[4] PHOTOACTIVABLE FLUOROPHORES .1. SYNTHESIS AND PHOTOACTIVATION OF ORTHO-NITROBENZYL-QUENCHED FLUORESCENT CARBAMATES
CUMMINGS, RT
KRAFFT, GA
[J]. TETRAHEDRON LETTERS, 1988, 29 (01) : 65 - 68
[5] PHOTOACTIVABLE FLUOROPHORES .2. SYNTHESIS AND PHOTOACTIVATION OF FUNCTIONALIZED 3-AROYL-2-(2-FURYL)-CHROMONES
CUMMINGS, RT
DIZIO, JP
KRAFFT, GA
[J]. TETRAHEDRON LETTERS, 1988, 29 (01) : 69 - 72
[6] A CALORIMETRIC DETERMINATION OF QUANTUM YIELD FOR IONIZATION OF MALACHITE GREEN CYANIDE BY ULTRAVIOLET RADIATION
FISHER, GJ
LEBLANC, JC
JOHNS, HE
[J]. PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1967, 6 (10) : 757 - &
[7] AZOBENZENE AS A CONVENIENT ACTINOMETER - EVALUATION VALUES FOR UV MERCURY LINES AND FOR THE N-2 LASER LINE
GAUGLITZ, G
HUBIG, S
[J]. JOURNAL OF PHOTOCHEMISTRY, 1981, 15 (03): : 255 - 257
[8] A NEW SENSITIVE CHEMICAL ACTINOMETER .2. POTASSIUM FERRIOXALATE AS A STANDARD CHEMICAL ACTINOMETER
HATCHARD, CG
PARKER, CA
[J]. PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES A-MATHEMATICAL AND PHYSICAL SCIENCES, 1956, 235 (1203): : 518 - 536
[9] DOSIMETRY IN PHOTOCHEMISTRY
JOHNS, HE
[J]. PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1968, 8 (06) : 547 - &
[10] PHOTOACTIVABLE FLUOROPHORES .3. SYNTHESIS AND PHOTOACTIVATION OF FLUOROGENIC DIFUNCTIONALIZED FLUORESCEINS
KRAFFT, GA
SUTTON, WR
CUMMINGS, RT
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1988, 110 (01) : 301 - 303

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