HIGH-LEVEL PRODUCTION OF BIOLOGICALLY-ACTIVE HUMAN CYTOSOLIC PHOSPHOLIPASE-A(2) IN BACULOVIRUS-INFECTED INSECT CELLS

被引：19

作者：

AMEGADZIE, BY

JIAMPETTI, D

CRAIG, RJ

APPELBAUM, E

SHATZMAN, AR

MAYER, RJ

DILELLA, AG

机构：

[1] SMITHKLINE BEECHAM PHARMACEUT,DEPT MOLEC GENET,KING OF PRUSSIA,PA 19406

[2] SMITHKLINE BEECHAM PHARMACEUT,DEPT MED CHEM,KING OF PRUSSIA,PA 19406

来源：

GENE | 1993年 / 128卷 / 02期

关键词：

PHOSPHOLIPIDS; ARACHIDONIC ACID; CDNA; EICOSANOIDS; INFLAMMATION;

D O I：

10.1016/0378-1119(93)90580-V

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Infection of Spodoptera frugiperda insect cells with a recombinant baculovirus expressing human cytosolic phospholipase A2 (cPLA2) resulted in the production of biologically active protein. The level of recombinant human cPLA2 production in infected insect cells was at least 50-fold higher than that observed in human monoblast U937 cells.

引用

页码：307 / 308

页数：2

共 10 条

[1] CHARACTERIZATION OF A 7-KILODALTON SUBUNIT OF VACCINIA VIRUS DNA-DEPENDENT RNA-POLYMERASE WITH STRUCTURAL SIMILARITIES TO THE SMALLEST SUBUNIT OF EUKARYOTIC RNA POLYMERASE-II
AMEGADZIE, BY
AHN, BY
MOSS, B
[J]. JOURNAL OF VIROLOGY, 1992, 66 (05) : 3003 - 3010
[2] A NOVEL ARACHIDONIC ACID-SELECTIVE CYTOSOLIC PLA2 CONTAINS A CA2+-DEPENDENT TRANSLOCATION DOMAIN WITH HOMOLOGY TO PKC AND GAP
CLARK, JD
LIN, LL
KRIZ, RW
RAMESHA, CS
SULTZMAN, LA
LIN, AY
MILONA, N
KNOPF, JL
[J]. CELL, 1991, 65 (06) : 1043 - 1051
[3] PURIFICATION OF A 110-KILODALTON CYTOSOLIC PHOSPHOLIPASE-A2 FROM THE HUMAN MONOCYTIC CELL-LINE U937
CLARK, JD
MILONA, N
KNOPF, JL
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (19) : 7708 - 7712
[4] EVOLUTIONARY RELATIONSHIPS AND IMPLICATIONS FOR THE REGULATION OF PHOSPHOLIPASE-A2 FROM SNAKE-VENOM TO HUMAN SECRETED FORMS
DAVIDSON, FF
DENNIS, EA
[J]. JOURNAL OF MOLECULAR EVOLUTION, 1990, 31 (03) : 228 - 238
[5] PURIFICATION OF A HIGH-MOLECULAR-MASS FORM OF PHOSPHOLIPASE-A2 FROM RAT-KIDNEY ACTIVATED AT PHYSIOLOGICAL CALCIUM CONCENTRATIONS
GRONICH, JH
BONVENTRE, JV
NEMENOFF, RA
[J]. BIOCHEMICAL JOURNAL, 1990, 271 (01) : 37 - 43
[6] PLATELET-ACTIVATING-FACTOR - A BIOLOGICALLY-ACTIVE PHOSPHOGLYCERIDE
HANAHAN, DJ
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1986, 55 : 483 - 509
[7] HOW IS THE LEVEL OF FREE ARACHIDONIC-ACID CONTROLLED IN MAMMALIAN-CELLS
IRVINE, RF
[J]. BIOCHEMICAL JOURNAL, 1982, 204 (01) : 3 - 16
[8] BACULOVIRUS EXPRESSION VECTORS WITH SINGLE-STRAND CAPABILITY
LIVINGSTONE, C
JONES, I
[J]. NUCLEIC ACIDS RESEARCH, 1989, 17 (06) : 2366 - 2366
[9] SHARP JD, 1991, J BIOL CHEM, V266, P14850
[10] SUMMERS MD, 1988, TEXAS AM AGR EXPT ST, V1555

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