ASSAY OF SUCCINATE-DEHYDROGENASE ACTIVITY BY A COLORIMETRIC-CONTINUOUS METHOD USING IODONITROTETRAZOLIUM CHLORIDE AS ELECTRON-ACCEPTOR

被引：114

作者：

MUNUJOS, P ^{[1
]}

COLLCANTI, J ^{[1
]}

GONZALEZSASTRE, F ^{[1
]}

GELLA, FJ ^{[1
]}

机构：

[1] UNIV AUTONOMA BARCELONA,HOSP ST PAU,SERV BIOQUIM,E-08025 BARCELONA,SPAIN

来源：

ANALYTICAL BIOCHEMISTRY | 1993年 / 212卷 / 02期

关键词：

D O I：

10.1006/abio.1993.1360

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A spectrophotometric assay method for determining succinate dehydrogenase activity is described in which iodonitrotetrazolium chloride is used as a final electron acceptor. The enzyme activity is determined by measuring the formation of formazan due to the tetrazolium salt reduction. The assay is continuous, rapid, simple, and sensitive, and may be used in the determination of enzyme activity either in tissue homogenates or as a marker of the mitochondrial fraction in cell fractionation procedures. © 1994 Academic Press, Inc. All rights reserved.

引用

页码：506 / 509

页数：4

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