ISOLATION OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME (PRRS) VIRUS IN A DANISH SWINE HERD AND EXPERIMENTAL-INFECTION OF PREGNANT GILTS WITH THE VIRUS

被引:108
作者
BOTNER, A [1 ]
NIELSEN, J [1 ]
BILLEHANSEN, V [1 ]
机构
[1] NATL VET LAB,DK-1790 COPENHAGEN V,DENMARK
关键词
PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS; PORCINE EPIDEMIC ABORTION AND RESPIRATORY SYNDROME; PIG; ISOLATION;
D O I
10.1016/0378-1135(94)90122-8
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The first case of porcine reproductive and respiratory syndrome (PRRS) in Denmark was diagnosed in March 1992 by the detection of specific antibodies against PRRS virus in serum samples originating from sows in a herd located on the island of Als. Subsequently, PRRS virus was isolated from a 200-sow farrow-to-finish herd with clinical signs consistent with PRRS. The virus was isolated by inoculation of pleural fluid from a stillborn piglet onto porcine pulmonary alveolar macrophages. The isolate was identified as PRRS virus by staining with a specific antiserum. By electron microscopy, the virus particle was found to be spherical and enveloped, measuring 45-55 nm in diameter and containing a 30-35 nm nucleocapsid. Only minor antigenic differences were found between the Danish and a Dutch isolate. Following intranasal inoculation of 3 pregnant gilts with the Danish isolate transplacental infection was demonstrated by the re-isolation of PRRS virus from approximately 45% of the piglets from the experimentally infected gilts. However, the experimental infection produced no significant reproductive disorders or other clinical signs. At autopsy, histopathological examination revealed slight interstitial pneumonia in a few piglets.
引用
收藏
页码:351 / 360
页数:10
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