CDNA AND DEDUCED AMINO-ACID-SEQUENCES OF A HUMAN COLON DIHYDRODIOL DEHYDROGENASE

被引：28

作者：

CIACCIO, PJ

TEW, KD

机构：

[1] Department of Pharmacology, Fox Chase Cancer Center, Philadelphia, PA 19111

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 1994年 / 1186卷 / 1-2期

关键词：

DIHYDRODIOL DEHYDROGENASE; COLON; CDNA SEQUENCE; (HUMAN);

D O I：

10.1016/0005-2728(94)90144-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 1.24 kbp cDNA encoding a human colon HT29 cell. dihydrodiol dehydrogenase of 323 amino acid residues (M(r) 36735) has been isolated and sequenced. The deduced amino acid sequence was 97%, 81%, and 76% identical to human liver dihydrodiol dehydrogenase (also called bile acid binder), human liver chlordecone reductase, and bovine lung prostanglandin f synthase, respectively. It was 98% identical in 990 bp overlap with the nucleotide sequence of human liver pseudochlordecone reductase. Northern blots revealed that dihydrodiol dehydrogenase(s) were markedly induced in HT29 colon cells by treatment with the Michael acceptor agent ethacrynic acid.

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页码：129 / 132

页数：4

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