OBSERVATION OF UNFREEZABLE WATER IN AQUEOUS-SOLUTION OF GLOBULE PROTEIN BY MICROWAVE DIELECTRIC MEASUREMENT

被引：45

作者：

MIURA, N

HAYASHI, Y

SHINYASHIKI, N

MASHIMO, S

机构：

[1] Department of Physics, Tokai University, Kanagawa, 259-12, Hiratsuka-shi

来源：

BIOPOLYMERS | 1995年 / 36卷 / 01期

关键词：

D O I：

10.1002/bip.360360103

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A freezing process analyzed by the dielectric method on aqueous solution of albumin has revealed water structure around protein molecule. A relaxation peak due to bound water attached on the protein surface around 100 MHz at room temperature was found. It could be seen commonly in globule proteins. Another peak due to a different kind of unfreezable water was found around 1 GHz at -6 degrees C. The amount of this water is estimated as 0.36 g water/g protein and in good agreement with that obtained by differential scanning calorimetry and nmr measurements. The water molecules form a shell layer around the protein molecule. (C) 1995 John Wiley & Sons, Inc.

引用

页码：9 / 16

页数：8

共 27 条

[1] CHANGES IN STATE OF WATER IN PROTEINACEOUS SYSTEMS [J].