BET3 ENCODES A NOVEL HYDROPHILIC PROTEIN THAT ACTS IN CONJUNCTION WITH YEAST SNARES

被引：62

作者：

ROSSI, G

KOLSTAD, K

STONE, S

PALLUAULT, F

FERRONOVICK, S

机构：

[1] YALE UNIV, SCH MED, HOWARD HUGHES MED INST, NEW HAVEN, CT 06510 USA

[2] YALE UNIV, SCH MED, DEPT CELL BIOL, NEW HAVEN, CT 06510 USA

来源：

MOLECULAR BIOLOGY OF THE CELL | 1995年 / 6卷 / 12期

关键词：

D O I：

10.1091/mbc.6.12.1769

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Here we report the identification of BET3, a new member of a group of interacting genes whose products have been implicated in the targeting and fusion of endoplasmic reticulum (ER) to Golgi transport vesicles with their acceptor compartment. A temperature-sensitive mutant in bet3-1 was isolated in a synthetic lethal screen designed to identify new genes whose products may interact with BET1, a type II integral membrane protein that is required for ER to Golgi transport. At 37 degrees C, bet3-1 fails to transport invertase, Ir-factor, and carboxypeptidase Y from the ER to the Golgi complex. As a consequence, this mutant accumulates dilated ER and small vesicles. The SNARE complex, a docking/fusion complex, fails to form in this mutant. Furthermore, BET3 encodes an essential 22-kDa hydrophilic protein that is conserved in evolution, which is not a component of this complex. These findings support the hypothesis that Bet3p may act before the assembly of the SNARE complex.

引用

页码：1769 / 1780

页数：12

共 35 条

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