ASSAY AND SUBSTRATE SPECIFICITY OF LIVER 2BETA-HYDROXY-STEROID DEHYDROGENASE

被引：11

作者：

KOERNER, DR

机构：

[1] Institute for Steroid Research, the Division of Neoplastic Medicine, Montefiore Hospital, New York

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 176卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0005-2760(69)90196-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

11β-Hydroxysteroid dehydrogenase activity of rat-liver microsomes has been assayed by a quantitative spectrophotometric method. The enzyme can be incubated at 45°, pH 10 for at least 45 min without loss of activity if NADP+ is included in the reaction mixture. Several 11β-hydroxysteroids are substrates of the enzyme but there is no activity towards 11β-hydroxysterone or 11β-hydroxysteroids with a C-21 sulfate, phosphate, acetate or hemisuccinate group. © 1969.

引用

页码：377 / &

共 9 条

[1] METABOLISM OF 11-OXYGENATED STEROIDS .2. 2-METHYL STEROIDS [J].