EVALUATION OF A GOLD-SILVER STAINING METHOD FOR DETECTION AND IDENTIFICATION OF CANDIDA SPECIES BY LIGHT-MICROSCOPY

被引：7

作者：

CAILLIEZ, JC ^{[1
]}

BOUDRISSA, A ^{[1
]}

MACKENZIE, DWR ^{[1
]}

POULAIN, D ^{[1
]}

机构：

[1] CENT PUBL HLTH LAB,MYCOL REFERENCE LAB,PUBL HLTH LAB SERV,LONDON NW9 5HT,ENGLAND

来源：

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 1990年 / 9卷 / 12期

关键词：

D O I：

10.1007/BF01967504

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A gold-silver staining procedure was evaluated for detection of Candida species of medical importance. Probes were prepared by coupling lectins or antibodies (polyclonal and monoclonal) directly or indirectly to colloidal gold particles. Structures reacting to these probes were specifically revealed by light microscopy in cells present in infected kidney tissue sections or in isolated yeast cells on glass slides. Definition, contrast and sensitivity were of a high order. Preliminary data showed that it was possible, using discriminating dilutions, to identify cells from different species of the genus Candida, grown in vitro, according to their ability to stain with polyclonal monospecific antisera. The advantages of gold-silver staining compared with other staining procedures currently used in routine mycological laboratories are its sensitivity, good definition, ease and rapidity, and long conservation of reaction. It is suggested that the procedure has applications for research and identification of yeasts in clinical samples.

引用

页码：886 / 891

页数：6

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