ISOLATION AND CHARACTERIZATION OF ACETONITRILE UTILIZING BACTERIA

被引：14

作者：

CHAPATWALA, KD

NAWAZ, MS

RICHARDSON, JD

WOLFRAM, JH

机构：

[1] Division of Natural Sciences, Selma University, Selma, AL

[2] Division of Biotechnology, INEL, EG and G, Idaho, Inc., Idaho Fall, ID

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY | 1990年 / 5卷 / 2-3期

关键词：

Acetonitrile utilizing bacteria; Chromobacterium; Pseudomonas aeruginosa;

D O I：

10.1007/BF01573854

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bacteria utilizing high concentrations of acetonitrile as the sole carbon source were isolated and identified as Chromobacterium sp. and Pseudomonas aeruginosa. Maximum growth was attained after 96 h of incubation and P. aeruginosa grew slightly faster than Chromobacterium sp. The strains were able to grow and oxidize acetonitrile at concentrations as high as 600 mM. However, higher concentrations inhibited growth and oxygen uptake. Degradation studies with (14C)acetonitrile indicated 57% of acetonitrile was degraded by Pseudomonas aeruginosa as compared to 43% by Chromobacterium. The isolates utilized different nitrile compounds as carbon substrates. © 1990 Society for Industrial Microbiology.

引用

页码：65 / 69

页数：5

共 15 条

[1]

Asano Y., Fujishiro K., Tani Y., Yamada H., Aliphatic nitrile hydratase from Arthrobacter sp. J-1. Purification and characterization, Agricultural and Biological Chemistry, 46, pp. 1165-1174, (1982)

[2]

Asano Y., Tachibana M., Tani Y., Yamada H., Purification and properties of an amidase which participates in nitrile degradation, Agricultural and Biological Chemistry, 46, pp. 1175-1181, (1982)

[3]

Asano Y., Yasuda Y., Tani Y., Yamada H., A new enzymatic method for acrylamide production, Agricultural and Biological Chemistry, 46, pp. 1183-89, (1982)

[4]

Bandyopadhyay A.K., Nagasawa T., Asano Y., Fujishiro K., Tani Y., Yamada H., Purification and characterization of benzonitrilases from Arthrobacter sp. Strain J-1, Appl. Environ. Microbiol., 31, pp. 900-906, (1986)

[5]

Bui K., Maestracci M., Thiery A., Arnaud A., Galzy P., A note on the enzymic action and biosynthesis of a nitrile-hydratase from a Brevibacterium sp, J. Appl. Bacteriol., 57, pp. 183-190, (1984)

[6]

Digeronimo M.J., Antoine A.D., Metabolism of acetonitrile and propionitrile by Nocardia rhodochrous LL100-21, Appl. Environ. Microbiol., 31, pp. 900-906, (1976)

[7]

Harper D.B., Microbial metabolism of aromatic nitriles, Biochem. J., 165, pp. 309-319, (1977)

[8]

Henahan J.F., Idol J.D., Setting the world of nitrile chemistry a fire, Chemical & Engineering News, 49, pp. 16-18, (1971)

[9]

Nagasawa T., Mathew C.D., Mauger J., Yamada H., Nitrile hydratase- catalysed production of nicotinamide from 3-Cyanopyridine in Rhodococcus rhodochrous J-1, Appl Environ Microbiol, 54, pp. 1766-69, (1988)

[10]

Thiery A., Maestracci M., Arnaud A., Galzy P., Nitriles as growth substrate for Brevibacterium sp. R312 and its mutant M2, Zentralbl. Mikrobiol., 141, pp. 575-582, (1986)

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