ACTIN-ENDOPLASMIC RETICULUM COMPLEXES IN DROSERA - THEIR STRUCTURAL RELATIONSHIP WITH THE PLASMALEMMA, NUCLEUS, AND ORGANELLES IN CELLS PREPARED BY HIGH-PRESSURE FREEZING

In epidermal cells of Drosera tentacles that have been preserved for ultrastructural analysis through high pressure freeze fixation and freeze substitution we describe the frequent occurrence of microfilament (MF)-endoplasmic reticulum (ER) complexes. These are found throughout the cytoplasm where they are observed in close association with the plasmalemma (PL), the tonoplast, nuclei, mitochondria, chloroplasts, and microbodies. The MF component of the complexes is identified as actin based on immunogold labelling with actin antibodies. The actin-ER complexes are prominent in the cortical cytoplasm. In this region a network of predominantly tubular ER occupies an intermediary position in which it associates closely with both the PL and the actin MFs. We suggest that the ER, especially those elements adjacent to the PL in the cortical cytoplasm, stabilizes the actin MFs and provides the necessary anchor against which the forces for cytoplasmic streaming are generated. © 1990 Springer-Verlag.