IMMUNOASSAYS FOR THE DETECTION OF HUMAN COLLAGENASE, STROMELYSIN, TISSUE INHIBITOR OF METALLOPROTEINASES (TIMP) AND ENZYME-INHIBITOR COMPLEXES

被引：89

作者：

COOKSLEY, S ^{[1
]}

HIPKISS, JB ^{[1
]}

TICKLE, SP ^{[1
]}

HOLMESIEVERS, E ^{[1
]}

DOCHERTY, AJP ^{[1
]}

MURPHY, G ^{[1
]}

LAWSON, ADG ^{[1
]}

机构：

[1] STRANGEWAYS RES LAB,DEPT CELL PHYSIOL,CAMBRIDGE CB1 4RN,ENGLAND

来源：

MATRIX | 1990年 / 10卷 / 05期

基金：

英国医学研究理事会;

关键词：

collagenase; ELISA; enzyme-inhibitor complexes; metalloproteinase; monoclonal antibody; stromelysin; TIMP;

D O I：

10.1016/S0934-8832(11)80183-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Immunoassays have been developed for human collagenase, stromelysin, tissue inhibitor of metalloproteinases (TIMP) and TIMP complexed with both of the active enzymes. Selection of antibodies of defined specificity enabled measurement of both the pro and active forms of the metalloproteinase. Free TIMP was quantified by the selection of a monoclonal antibody which did not recognise TIMP when complexed with metalloproteinases. Detection of enzyme-inhibitor complexes was achieved by capturing the TIMP component of the complex and revealing the metalloenzyme using specific antibodies. © 1990, Gustav Fischer Verlag · Stuttgart · New York. All rights reserved.

引用

页码：285 / 291

页数：7

共 25 条

[1] ABRAMS DC, 1990, ADV LABORATORY AUTOM, V6, P369
[2] Bos E S, 1981, J Immunoassay, V2, P187, DOI 10.1080/15321818108056977
[3] THE INTERACTION OF PURIFIED RABBIT BONE COLLAGENASE WITH PURIFIED RABBIT BONE METALLOPROTEINASE INHIBITOR
CAWSTON, TE
MURPHY, G
MERCER, E
GALLOWAY, WA
HAZLEMAN, BL
REYNOLDS, JJ
[J]. BIOCHEMICAL JOURNAL, 1983, 211 (02) : 313 - 318
[4] CAWSTON TE, 1981, METHOD ENZYMOL, V80, P711
[5] COLLIER IE, 1988, J BIOL CHEM, V263, P6579
[6] EVIDENCE FOR METALLOPROTEINASE AND METALLOPROTEINASE INHIBITOR IMBALANCE IN HUMAN OSTEOARTHRITIC CARTILAGE
DEAN, DD
MARTELPELLETIER, J
PELLETIER, JP
HOWELL, DS
WOESSNER, JF
[J]. JOURNAL OF CLINICAL INVESTIGATION, 1989, 84 (02) : 678 - 685
[7] SYNOVIAL-FLUID COLLAGENASE IN PATIENTS WITH DESTRUCTIVE ARTHRITIS OF THE SHOULDER JOINT
DIEPPE, PA
CAWSTON, T
MERCER, E
CAMPION, GV
HORNBY, J
HUTTON, CW
DOHERTY, M
WATT, I
WOOLF, AD
HAZLEMAN, B
[J]. ARTHRITIS AND RHEUMATISM, 1988, 31 (07): : 882 - 890
[8] SEQUENCE OF HUMAN-TISSUE INHIBITOR OF METALLOPROTEINASES AND ITS IDENTITY TO ERYTHROID-POTENTIATING ACTIVITY
DOCHERTY, AJP
LYONS, A
SMITH, BJ
WRIGHT, EM
STEPHENS, PE
HARRIS, TJR
MURPHY, G
REYNOLDS, JJ
[J]. NATURE, 1985, 318 (6041) : 66 - 69
[9] ANTIBODIES TO MAJOR HISTOCOMPATIBILITY ANTIGENS PRODUCED BY HYBRID CELL LINES
GALFRE, G
HOWE, SC
MILSTEIN, C
BUTCHER, GW
HOWARD, JC
[J]. NATURE, 1977, 266 (5602) : 550 - 552
[10] PURIFICATION AND CHARACTERIZATION OF A RABBIT BONE METALLOPROTEINASE THAT DEGRADES PROTEOGLYCAN AND OTHER CONNECTIVE-TISSUE COMPONENTS
GALLOWAY, WA
MURPHY, G
SANDY, JD
GAVRILOVIC, J
CAWSTON, TE
REYNOLDS, JJ
[J]. BIOCHEMICAL JOURNAL, 1983, 209 (03) : 741 - 752

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