GENETIC-TRANSFORMATION OF VIBRIO-PARAHAEMOLYTICUS, VIBRIO-ALGINOLYTICUS, AND VIBRIO-CHOLERAE NON-0-1 WITH PLASMID DNA BY ELECTROPORATION

被引：20

作者：

HAMASHIMA, H ^{[1
]}

NAKANO, T ^{[1
]}

TAMURA, S ^{[1
]}

ARAI, T ^{[1
]}

机构：

[1] SHOWA COLL PHARMACEUT SCI,DEPT MICROBIOL,MACHIDA,TOKYO 194,JAPAN

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1990年 / 34卷 / 08期

关键词：

D O I：

10.1111/j.1348-0421.1990.tb01047.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

An electroporation procedure for the plasmid-mediated transformation of the genus Vibrio was performed, as part of an effort to develop recombinant DNA techniques for genetic manipulation of the genus Vibrio. Vibrio parahaemolyticus, V. alginolyticus, and V. cholerae non O–1 (9 different strains) were transformed with 3 vector plasmids (pACYC184, pHSG398, and pBR325). The efficiency of transformation was highly dependent on three parameters: the concentration of plasmid DNA; the strength of the electric field; and the combination of plasmid DNA and recipient strain. The drug-resistance genes on the vector plasmid were expressed in the Vibrio strains. © 1990, Center For Academic Publications Japan. All rights reserved.

引用

页码：703 / 708

页数：6

共 22 条

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