ELECTROOXIDATION OF NADH WITH CO(PHEN)3(3+) BY NADH DEHYDROGENASE PURIFIED FROM BACILLUS-STEAROTHERMOPHILUS

被引:25
作者
MATSUE, T
YAMADA, H
CHANG, HC
UCHIDA, I
机构
[1] Department of Molecular Chemistry and Engineering, Faculty of Engineering, Tohoku University, Sendai
来源
BIOELECTROCHEMISTRY AND BIOENERGETICS | 1990年 / 24卷 / 03期
基金
日本学术振兴会;
关键词
D O I
10.1016/0302-4598(90)80034-G
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The NADH dehydrogenase purified from Bacillus stearothermophilus catalyzes effectively the electro-chemical oxidation of NADH mediated by Co(phen)3(3+). Stopped-flow measurements indicate that the rate constant for the oxidation of the reduced enzyme by Co(phen)3(3+) is 5 x 10(6) M-1 s-1 and that for the oxidation of NADH by the oxidized enzyme > 3 x 10(7) M-1 s-1. The voltammograms, showing catalytic prepeaks, were simulated using the above rate constants.
引用
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页码:347 / 354
页数:8
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