GENE-EXPRESSION OF INTERLEUKIN-1-BETA DURING HEMODIALYSIS

It is still controversial whether the hemodialysis (HD) procedure is an inflammatory process in vivo. Therefore, we studied the gene expression of interleukin-1beta (IL-1beta) as a marker of inflammation in peripheral blood mononuclear cells (PBMC) of patients during HD by Northem blotting and polymerase chain reaction. Compared to PBMC separated pre-HD (1.0 densitometric units), the amount of IL-1beta mRNA was increased in PBMC leaving the dialyzer (12.2 +/- 2 densitometric units, P < 0.01), but was not increased in PBMC re-entering the dialyzer from the systemic circulation (0.6 +/- 0.1 densitometric units) in all 12 patients studied. The maximal amount of IL-1beta mRNA in PBMC was seen at five minutes after start of HD. There was a significant correlation between the increase in IL-1beta mRNA and the increase in activated complement C5a (r = 0.71, P < 0.01). HD using less complement-activating membranes (hemophan, polysulfone, polyamide or polyacrylonitrile) resulted in no detectable IL-1beta mRNA. Furthermore, a monoclonal antibody against human C5a reduced the increase in IL-1beta mRNA by 83% (P < 0.05), indicating that C5a plays a major role for induction of IL-1beta mRNA during HD. This study demonstrates that during HD with regenerated cellulose, gene expression for IL-1beta takes place in PBMC.