ACCESSIBILITY OF MITOCHONDRIAL KYNURENINE HYDROXYLASE REACTION TO EXOGENOUS AND ENDOGENOUS PYRIDINE NUCLEOTIDES

被引：10

作者：

CAMMER, W

MOORE, CL

机构：

[1] Albert Einstein College of Medicine, Yeshiva University, The Saul R. Korey Department of Neurology, Bronx, NY

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1969年 / 134卷 / 02期

关键词：

D O I：

10.1016/0003-9861(69)90287-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The outer and inner membranes of rat liver mitochondria were separated, and it was confirmed that the kynurenine hydroxylase is located in the outer membrane. In intact mitochondria the kynurenine hydroxylase could be supported by exogenous NADPH. NADPH generated from endogenous mitochondrial NADP+ by the isocitric dehydrogenase reaction could not be used for the kynurenine hydroxylase reaction unless the mitochondria were solubilized by detergent. Conversely, NADP+ generated by the kynurenine hydroxylase from exogenous NADPH could not be reduced by the intramitochondrial isocitric dehydrogenase unless the mitochondria were solubilized. These results provide direct evidence of a permeability barrier for pyridine nucleotides between the outer mitochondrial membrane and the inner membrane compartment. © 1969.

引用

页码：290 / &

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