CONFORMATIONAL-ANALYSIS OF 1-ALPHA,25-DIHYDROXYVITAMIN-D3 BY NUCLEAR-MAGNETIC-RESONANCE

被引：32

作者：

EGUCHI, T

IKEKAWA, N

机构：

[1] Department of Fundamental Science, Iwaki Meisei University, Iwaki, Fukushima

来源：

BIOORGANIC CHEMISTRY | 1990年 / 18卷 / 01期

关键词：

D O I：

10.1016/0045-2068(90)90012-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The conformational equilibrium of 1α,25-dihydroxyvitamin D3 (3) was studied by variable temperature 1H and 13C NMR spectroscopy. At temperatures below -100°C, two conformations in a ratio of 55: 45 were observed by splitting of the signals for H-3α, H-4α, and H-6. A free enthalpy of interconversion was estimated to be ΔG193°K‡, 39.6 kJ/mol from the 13C NMR spectrum at -80°C. From an analysis of the A-ring coupling constants in various solvents, we propose that the A-ring of 3 undergoes chair-chair interconversion in hydrophobic media and chair-half-chair interconversion in hydrophilic media. © 1990.

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页码：19 / 29

页数：11

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