SYNERGISTIC EFFECT OF MAGNESIUM AND CALCIUM-IONS IN THE ACTIVATION OF PHOSPHOLIPASE-A2 OF LIVER MACROPHAGES

被引：20

作者：

KRAUSE, H

DIETER, P

SCHULZESPECKING, A

BALLHORN, A

FERBER, E

DECKER, K

机构：

[1] UNIV FREIBURG, INST BIOCHEM, W-7800 FREIBURG, GERMANY

[2] MAX PLANCK INST IMMUNBIOL, W-7800 FREIBURG, GERMANY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 175卷 / 02期

关键词：

D O I：

10.1016/0006-291X(91)91597-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In cell-free extracts of rat liver macrophages (Kupffer cells) phospholipase A2 was found to be strongly activated at free Ca2+ concentrations from 100 nM to 1 μM in the presence of 4 mM free Mg2+. This is within the range of intracellular free Ca2+ reported for basal and various stimulated conditions, respectively. Ca2+ alone increased phospholipase A2 activity at high Ca2+ concentrations (1mM) whereas Mg2+ alone had only little stimulatory effect. Calmodulin does not seem to participate in the regulation of phospholipase A2 although it relieved the inhibition of phospholipase A2 activity by calmodulin antagonists. © 1991.

引用

页码：532 / 536

页数：5

共 19 条

[1] A SIMPLE METHOD FOR THE ACCURATE DETERMINATION OF FREE [CA] IN CA-EGTA SOLUTIONS [J].