BLEOMYCIN-TREATED DNA IS SPECIFICALLY CLEAVED ONLY BY ENDONUCLEASE-IV IN ESCHERICHIA-COLI

被引：6

作者：

HAGENSEE, ME ^{[1
]}

MOSES, RE ^{[1
]}

机构：

[1] BAYLOR UNIV,DEPT CELL BIOL,1 BAYLOR PLAZA,HOUSTON,TX 77030

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1048卷 / 01期

关键词：

(E. coli); AP endonuclease; Bleomycin; Endonuclease IV;

D O I：

10.1016/0167-4781(90)90016-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated an endonuclease from E. coli active on bleomycin-treated DNA. Purification on DEAE-cellulose separated this activity in strains lacking endonuclease I, endonuclease III or exonuclease III. After DEAE chromatography, the enzyme was active in the absence of divalent cations and was not inhibited by tRNA or harmane. In addition, this enzyme was stable at 45°C for 20 min. These properties are consistent with this activity being endonuclease IV. This was supported by our finding no activity in a strain lacking endonuclease IV. © 1990.

引用

页码：19 / 23

页数：5

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