A SIMPLE ASSAY FOR PROTEOLYSIS OF IGFBP-3

被引：173

作者：

LAMSON, G

GIUDICE, LC

ROSENFELD, RG

机构：

[1] STANFORD UNIV, MED CTR, DEPT PEDIAT, DIV ENDOCRINOL, STANFORD, CA 94305 USA

[2] STANFORD UNIV, MED CTR, DEPT OBSTET & GYNECOL, DIV REPROD ENDOCRINOL, STANFORD, CA 94305 USA

来源：

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM | 1991年 / 72卷 / 06期

关键词：

D O I：

10.1210/jcem-72-6-1391

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

An assay for assessing the proteolysis of IGFBP-3 by a biological fluid or protein has been developed using I-125 hIGFBP-3 recombinantly derived from Escherichia coli as a substrate indicator. The labelled substrate is incubated at 37-degrees in the presence of the agent being tested and proteolysis is visualized by SDS-PAGE and autoradiography. The assay showed that the pregnancy associated protease is Ca++ dependent. The substrate is also proteolyzed by rat and mouse pregnancy sera, making the assay applicable to studies in those animal systems.

引用

页码：1391 / 1393

页数：3

共 9 条

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