INTERCALATION OF ANTHRACYCLINES INTO LIVING CELL-DNA ANALYZED BY FLOW-CYTOMETRY

被引:32
作者
BELLOC, F [1 ]
LACOMBE, F [1 ]
DUMAIN, P [1 ]
LOPEZ, F [1 ]
BERNARD, P [1 ]
BOISSEAU, MR [1 ]
REIFERS, J [1 ]
机构
[1] HOP HAUT LEVEQUE,SERV GREFFE MOELLE OSSEUSE,F-33604 PESSAC,FRANCE
来源
CYTOMETRY | 1992年 / 13卷 / 08期
关键词
MULTIDRUG RESISTANCE; FLUORESCENCE RESONANCE ENERGY TRANSFER; HOECHST; 33342; DAUNORUBICIN; IDARUBICIN;
D O I
10.1002/cyto.990130811
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Anthracyclines (ANT) are used in the treatment of leukemia and other cancers. These drugs have been shown to intercalate between the strands of DNA. In the present study, we show that the amount of ANT intercalated into DNA can be determined by measuring the fluorescence resonance energy transfer (FRET) between Hoechst 33342 (H33342) and ANT bound to DNA. The transfer efficiency was found to depend on the amount of disposable ANT but was independent of the amount of H33342 bound to DNA over a wide range of H33342 concentrations. The method was adapted for flow cytometric measurement of FRET in whole living cells and was used to evaluate the degree of intercalation of daunorubicin (DAU) and idarubicine (IDA) into DAU-sensitive and DAU-resistant leukemic cell lines. ANT intercalation into DNA was affected by factors which modify the intracytoplasmic concentration of ANT, and it was shown that the action of ANT and the resistance to ANT could not be attributed solely to the intercalative effect of the drugs. The method has advantages over previously described methods and represents a useful complementary tool in studies on the mode of action of ANT and the mechanisms of chemoresistance.
引用
收藏
页码:880 / 885
页数:6
相关论文
共 32 条
  • [1] BACHUR NR, 1978, CANCER RES, V38, P1745
  • [2] P388 LEUKEMIA-CELLS RESISTANT TO THE ANTHRACYCLINE MENOGARIL LACK MULTIDRUG RESISTANT PHENOTYPE
    BADINER, GJ
    MOY, BC
    SMITH, KS
    TARPLEY, WG
    GROPPI, VE
    BHUYAN, BK
    [J]. BRITISH JOURNAL OF CANCER, 1990, 62 (03) : 378 - 384
  • [3] DAUNORUBICIN INHIBITION OF DNA-DEPENDENT RNA-POLYMERASES FROM EHRLICH ASCITES TUMOR-CELLS
    BARTHELEMYCLAVEY, V
    MOLINIER, C
    AUBELSADRON, G
    MARAL, R
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1976, 69 (01): : 23 - 33
  • [4] BURRES NS, 1988, CANCER BIOCHEM BIOPH, V10, P47
  • [5] ON PHYSICO-CHEMICAL INTERACTIONS BETWEEN DAUNOMYCIN AND NUCLEIC ACIDS
    CALENDI, E
    DIMARCO, A
    REGGIANI, M
    SCARPINATO, B
    VALENTINI, L
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1965, 103 (01) : 25 - +
  • [6] SEQUENCE-SELECTIVE TOPOISOMERASE-II INHIBITION BY ANTHRACYCLINE DERIVATIVES IN SV40 DNA - RELATIONSHIP WITH DNA-BINDING AFFINITY AND CYTOTOXICITY
    CAPRANICO, G
    ZUNINO, F
    KOHN, KW
    POMMIER, Y
    [J]. BIOCHEMISTRY, 1990, 29 (02) : 562 - 569
  • [7] CAPRANICO G, 1987, CANCER RES, V47, P3752
  • [8] IMPROVED CELLULAR ACCUMULATION IS CHARACTERISTIC OF ANTHRACYCLINES WHICH RETAIN HIGH-ACTIVITY IN MULTIDRUG RESISTANT CELL-LINES, ALONE OR IN COMBINATION WITH VERAPAMIL OR CYCLOSPORINE-A
    COLEY, HM
    TWENTYMAN, PR
    WORKMAN, P
    [J]. BIOCHEMICAL PHARMACOLOGY, 1989, 38 (24) : 4467 - 4475
  • [9] MATHEMATICAL-ANALYSIS OF DNA DISTRIBUTIONS DERIVED FROM FLOW MICROFLUOROMETRY
    DEAN, PN
    JETT, JH
    [J]. JOURNAL OF CELL BIOLOGY, 1974, 60 (02) : 523 - 527
  • [10] DEISABELLA P, 1990, MOL PHARMACOL, V37, P11