DETECTION OF VARIATION IN THE RIBOSOMAL-RNA GENE CLUSTERS BY A MODIFIED FLUORESCENCE INSITU HYBRIDIZATION METHOD

被引：5

作者：

FUKUYAMA, R

SHIMIZU, N

机构：

[1] Department of Molecular Biology, Keio University School of Medicine, Tokyo, 160, 35 Shinanomachi, Shinjuku-ku

来源：

JAPANESE JOURNAL OF HUMAN GENETICS | 1992年 / 37卷 / 02期

关键词：

FISH; TEXAS RED; Q-BANDING; RIBOSOMAL-RNA GENE; GENE MAPPING;

D O I：

10.1007/BF01899735

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Physical mapping of genes by fluorescence in situ hybridization (FISH) has become routine using fluorescein isothiocyanate (FITC) for probe detection and propidium iodide (PI) for chromosome staining. We have modified this conventional FISH method in a way that utilizes Texas red (TR) for signal detection and quinacrine mustard (QM) for chromosome banding. Using this Texas red and quinacrine (TRQ) method, we were able to identify individual acrocentric chromosomes with varying degrees of ribosomal RNA gene clusters. Two acrocentric chromosomes were found to carry extremely small number of rRNA gene copies as compared to the other eight counterparts in human diploid lymphoblastoid cell line GM00130B. Thus, the TRQ method allows one to probe for a specific sequence while identifying individual chromosomes and will be powerful for the chromosomal localization of various genes.

引用

页码：139 / 143

页数：5

共 14 条

[1]

BHATT B, 1990, PRINCIPLE PRACTICE, P149

[2]

CASPERSSON T, 1971, HEREDITAS-GENETISK A, V67, P89

[3] SIMULTANEOUS LOCALIZATION OF COSMIDS AND CHROMOSOME R-BANDING BY FLUORESCENCE MICROSCOPY - APPLICATION TO REGIONAL MAPPING OF HUMAN CHROMOSOME-11 [J].

CHERIF, D ;

JULIER, C ;

DELATTRE, O ;

DERRE, J ;

LATHROP, GM ;

BERGER, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (17) :6639-6643

[4] THE POLYMORPHIC HUMAN DNA-SEQUENCE D8S8 ASSIGNED TO 8Q13-21.1, CLOSE TO THE CARBONIC-ANHYDRASE GENE-CLUSTER, BY ISOTOPIC AND NONISOTOPIC INSITU HYBRIDIZATION AND BY LINKAGE ANALYSIS [J].

EDWARDS, Y ;

WILLIAMS, S ;

WEST, L ;

LIPOWICZ, S ;

SHEER, D ;

ATTWOOD, J ;

SPURR, N ;

SARKAR, R ;

SAHA, N ;

POVEY, S .

ANNALS OF HUMAN GENETICS, 1990, 54 :131-+

[5] RELATIONSHIP BETWEEN PRODUCTION OF EPIDERMAL GROWTH-FACTOR RECEPTORS, GENE AMPLIFICATION, AND CHROMOSOME-7 TRANSLOCATION IN VARIANT-A431 CELLS [J].

GILL, GN ;

WEBER, W ;

THOMPSON, DM ;

LIN, C ;

EVANS, RM ;

ROSENFELD, MG ;

GAMOU, S ;

SHIMIZU, N .

SOMATIC CELL AND MOLECULAR GENETICS, 1985, 11 (04) :309-318

[6] HIGH-RESOLUTION MAPPING OF HUMAN CHROMOSOME-11 BY INSITU HYBRIDIZATION WITH COSMID CLONES [J].

LICHTER, P ;

TANG, CJC ;

CALL, K ;

HERMANSON, G ;

EVANS, GA ;

HOUSMAN, D ;

WARD, DC .

SCIENCE, 1990, 247 (4938) :64-69

[7] ISOLATION OF GIANT DNA FRAGMENTS FROM FLOW-SORTED HUMAN-CHROMOSOMES [J].

MINOSHIMA, S ;

KAWASAKI, K ;

FUKUYAMA, R ;

MAEKAWA, M ;

KUDOH, J ;

SHIMIZU, N .

CYTOMETRY, 1990, 11 (04) :539-546

[8] INHERITANCE OF RDNA SPACER LENGTH VARIANTS IN MAN [J].

RANZANI, GN ;

BERNINI, LF ;

CRIPPA, M .

MOLECULAR AND GENERAL GENETICS, 1984, 196 (01) :141-145

[9] MOLECULAR-CLONING AND CHROMOSOMAL ASSIGNMENT OF A HUMAN PERFORIN (PFP) GENE [J].

SHINKAI, Y ;

YOSHIDA, MC ;

MAEDA, K ;

KOBATA, T ;

MARUYAMA, K ;

YODOI, J ;

YAGITA, H ;

OKUMURA, K .

IMMUNOGENETICS, 1989, 30 (06) :452-457

[10] CHROMOSOME MAPPING OF THE HUMAN CYTIDINE-5'-TRIPHOSPHATE SYNTHETASE (CTPS) GENE TO BAND 1P34.1-P34.3 BY FLUORESCENCE INSITU HYBRIDIZATION [J].

TAKAHASHI, E ;

YAMAUCHI, M ;

TSUJI, H ;

HITOMI, A ;

MEUTH, M ;

HORI, T .

HUMAN GENETICS, 1991, 88 (01) :119-121

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