BIOLOGICALLY-ACTIVE OLIGODEOXYRIBONUCLEOTIDES .2. STRUCTURE-ACTIVITY-RELATIONSHIPS OF ANTI-HIV-1 PENTADECADEOXYRIBONUCLEOTIDES BEARING 5'-END-MODIFICATIONS

被引：24

作者：

HOTODA, H

MOMOTA, K

FURUKAWA, H

KANEKO, M

NAKAMURA, T

KIMURA, S

SHIMADA, K

机构：

[1] SANKYO CO LTD,BIOL RES LABS,SHINAGAWA KU,TOKYO 140,JAPAN

[2] SANKYO CO LTD,ANALYT & METAB RES LABS,SHINAGAWA KU,TOKYO 140,JAPAN

[3] UNIV TOKYO,INST MED SCI,DEPT INFECT DIS,MINATO KU,TOKYO 108,JAPAN

来源：

NUCLEOSIDES & NUCLEOTIDES | 1994年 / 13卷 / 6-7期

关键词：

D O I：

10.1080/15257779408012159

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

5'-End-modified pentadecadeoxyribonucleotides (15mers) with a sequence complementary to the tat 2nd splicing acceptor region of human immunodeficiency virus type 1 (HIV-1) were prepared and evaluated for anti-HIV-1 activity. The structures of modified 15mers were confirmed by negative ion LSI mass spectroscopy, and the anti-HIV-1 activities were evaluated in vitro by MTT assay using MT-4 cells. While the unmodified 15mer had no activity in our assay system, the 15mers bearing modifications with trityl-type substituents at the 5'-end showed potent anti-HIV-1 activities.

引用

页码：1375 / 1395

页数：21

共 22 条

[1] ANTISENSE PHOSPHOROTHIOATE OLIGODEOXYNUCLEOTIDES TARGETED TO THE VPR GENE INHIBIT HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REPLICATION IN PRIMARY HUMAN MACROPHAGES [J].