QUANTITATION OF RNA USING THE POLYMERASE CHAIN-REACTION

被引：219

作者：

FOLEY, KP ^{[1
]}

LEONARD, MW ^{[1
]}

ENGEL, JD ^{[1
]}

机构：

[1] NORTHWESTERN UNIV,DEPT BIOCHEM MOLECULAR BIOL & CELL BIOL,EVANSTON,IL 60208

来源：

TRENDS IN GENETICS | 1993年 / 9卷 / 11期

关键词：

D O I：

10.1016/0168-9525(93)90137-7

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Sequential use of reverse transcriptase and the polymerase chain reaction (RT-PCR) permits rapid and sensitive detection of specific RNAs. However, the greatest advantage of RT-PCR, its remarkable sensitivity, has also limited its usefulness in quantitative applications, since the effects of minor variations in reaction conditions from sample to sample are greatly magnified during the amplification process. Several recently developed techniques circumvent this problem, allowing accurate quantitation of RNA using RT-PCR.

引用

页码：380 / 385

页数：6

共 38 条

[1] ABSOLUTE MESSENGER-RNA QUANTIFICATION USING THE POLYMERASE CHAIN-REACTION (PCR) - A NOVEL-APPROACH BY A PCR AIDED TRANSCRIPT TITRATION ASSAY (PATTY) [J].

BECKERANDRE, M ;

HAHLBROCK, K .

NUCLEIC ACIDS RESEARCH, 1989, 17 (22) :9437-9446

[2] CELLULAR-LOCALIZATION AND HORMONAL-REGULATION OF FOLLICLE-STIMULATING-HORMONE AND LUTEINIZING-HORMONE RECEPTOR MESSENGER-RNAS IN THE RAT OVARY [J].

CAMP, TA ;

RAHAL, JO ;

MAYO, KE .

MOLECULAR ENDOCRINOLOGY, 1991, 5 (10) :1405-1417

[3] QUANTITATIVE ESTIMATION OF MINOR MESSENGER-RNAS BY CDNA-POLYMERASE CHAIN-REACTION - APPLICATION TO DYSTROPHIN MESSENGER-RNA IN CULTURED MYOGENIC AND BRAIN-CELLS [J].

CHELLY, J ;

MONTARRAS, D ;

PINSET, C ;

BERWALDNETTER, Y ;

KAPLAN, JC ;

KAHN, A .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 187 (03) :691-698

[4] TRANSCRIPTION OF THE DYSTROPHIN GENE IN HUMAN-MUSCLE AND NON-MUSCLE TISSUES [J].

CHELLY, J ;

KAPLAN, JC ;

MAIRE, P ;

GAUTRON, S ;

KAHN, A .

NATURE, 1988, 333 (6176) :858-860

[5]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[6]

Clementi M, 1993, PCR Methods Appl, V2, P191

[7]

Dilworth D D, 1992, PCR Methods Appl, V1, P279

[8]

Ferre F, 1992, PCR Methods Appl, V2, P1

[9] INDIVIDUAL STAGE SELECTOR ELEMENT MUTATIONS LEAD TO RECIPROCAL CHANGES IN BETA-GLOBIN VS EPSILON-GLOBIN GENE-TRANSCRIPTION - GENETIC CONFIRMATION OF PROMOTER COMPETITION DURING GLOBIN GENE SWITCHING [J].

FOLEY, KP ;

ENGEL, JD .

GENES & DEVELOPMENT, 1992, 6 (05) :730-744

[10] ANALYSIS OF CYTOKINE MESSENGER-RNA AND DNA - DETECTION AND QUANTITATION BY COMPETITIVE POLYMERASE CHAIN-REACTION [J].

GILLILAND, G ;

PERRIN, S ;

BLANCHARD, K ;

BUNN, HF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (07) :2725-2729

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