MOLECULAR MIMICRY AND ANKYLOSING-SPONDYLITIS - POSSIBLE ROLE OF A NOVEL SEQUENCE IN PULLULANASE OF KLEBSIELLA-PNEUMONIAE

被引：78

作者：

FIELDER, M

PIRT, SJ

TARPEY, I

WILSON, C

CUNNINGHAM, P

ETTELAIE, C

BINDER, A

BANSAL, S

EBRINGER, A

机构：

[1] UNIV LONDON KINGS COLL, DIV LIFE SCI, INFECT & IMMUN GRP, LONDON W8 7AH, ENGLAND

[2] UNIV LONDON KINGS COLL, DEPT COMP, LONDON W8 7AH, ENGLAND

[3] ROYAL FREE HOSP, DEPT CHEM & BIOCHEM, LONDON NW3 2QG, ENGLAND

[4] LISTER HOSP, DEPT RHEUMATOL, STEVENAGE, HERTS, ENGLAND

[5] UNIV LONDON KINGS COLL, DEPT PHARM, LONDON W8 7AH, ENGLAND

[6] UCL, MIDDLESEX HOSP, SCH MED, DEPT RHEUMATOL, LONDON, ENGLAND

来源：

FEBS LETTERS | 1995年 / 369卷 / 2-3期

基金：

英国生物技术与生命科学研究理事会;

关键词：

ANKYLOSING SPONDYLITIS; PULLULANASE; NOVEL SEQUENCE IN PULLULANASE; MOLECULAR MIMICRY; KLEBSIELLA PNEUMONIAE;

D O I：

10.1016/0014-5793(95)00760-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Molecular mimicry has been shown between two sequences of Klebsiella pneumoniae pulD secretion protein (DRDE) with HLA-B27 (DRED) and pulA (pullulanase) enzyme (Gly-X-Pro) with types I, III and IV collagen respectively. IgG antibody levels in AS patients were elevated against 16mer synthetic peptides of HLA-B27 and pulD by enzyme linked immunosorbent assay (ELISA) compared to controls (P < 0.001). ELISA assays against K. pneumoniae grown in the absence and presence of pullulan demonstrated significant levels of IgA antibody in AS patients compared to controls (P < 0.001). Increased IgA and IgG antibody levels to pulA and types I and IV collagen were observed in AS patients compared to controls (P < 0.001). These observations could be relevant in the sequence of molecular events in AS.

引用

页码：243 / 248

页数：6

共 18 条

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