ADDITIVE EFFECT OF ACETAZOLAMIDE AND OUABAIN ON PANCREATIC SECRETION IN VITRO

Basal fluid and electrolyte secretion of the rabbit pancreas were studied in vitro. Acetazolamide added to the bathing solution in 0.3×10-3 to 3×10-3 M concentration reduced flow, with a maximal inhibition of 25-27% reached at 10-3-3×10-3 M diamox. Ouabain also inhibited pancreatic fluid secretion in vitro, 28% flow inhibition being observed after addition of 10-6 M ouabain. When both drugs were added together in these concentrations to the bathing solution a maximal average flow inhibition of 43% was observed, significantly higher than for each drug alone. This indicates that the effects of the drugs are additive and suggests that ouabain and acetazolamide act on different systems in the cells involved in the secretion of pancreatic fluid. © 1969 Springer-Verlag.