THE INTRINSIC FLUORESCENCE OF THE RECOMBINANT HUMAN-LEUKOCYTE INTERFERON-ALPHA-A AND FIBROBLAST INTERFERON-BETA-1

被引：3

作者：

BORUKHOV, SI

STRONGIN, AY

机构：

[1] Institute of Molecular Genetics, USSR Academy of Sciences, Kurchatov sq.

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 169卷 / 01期

关键词：

D O I：

10.1016/0006-291X(90)91465-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The environment of Trp residues of the recombinant human interferons has been studied by the analysis of the emission spectra of native and denatured proteins at pH 1,5-8,5 and temperature 10-65°C in the presence and absence of the anionic, cationic and neutral to charge contact quenchers-KI, CsCl and acrylamide, respectively. The obtained data allow to suppose that in IFN-αA and IFN-β1 Trp141 interacts with Arg145 and one or several from the following residues-Phe124, Ile127, Tyr130, Leu131, whereas Trp77 - with Arg33 and Phe36, Phe78, Leu81 or Leu82 (Ile81 or Val82 for IFN-β1). © 1990.

引用

页码：282 / 288

页数：7

共 16 条

[1]

BORUKHOV S I, 1988, Biokhimiya, V53, P1718

[2]

BORUKHOV SI, 1990, IN PRESS BIOCH BIOPH

[3] FLUORESCENCE QUENCHING STUDIES WITH PROTEINS [J].