SYNTHETIC DNA PROBES FOR THE IDENTIFICATION OF SIBLING SPECIES IN THE ANOPHELES-GAMBIAE COMPLEX

被引：18

作者：

HILL, SM ^{[1
]}

URWIN, R ^{[1
]}

KNAPP, TF ^{[1
]}

CRAMPTON, JM ^{[1
]}

机构：

[1] UNIV LIVERPOOL,LIVERPOOL SCH TROP MED,WOLFSON UNIT MOLEC GENET,PEMBROKE PL,LIVERPOOL L3 5QA,ENGLAND

来源：

MEDICAL AND VETERINARY ENTOMOLOGY | 1991年 / 5卷 / 04期

关键词：

ANOPHELES-GAMBIAE COMPLEX; SPECIES IDENTIFICATION; OLIGONUCLEOTIDE PROBES; CONSENSUS SEQUENCE; DOT-BLOTS; SQUASH-BLOTS;

D O I：

10.1111/j.1365-2915.1991.tb00574.x

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

The cloned DNA sequences pAna1, pAnq1 and pAnml4, which may be used to distinguish between at least five of the six species in the Anopheles gambiae Giles complex of Afrotropical malaria vector mosquitoes, have been sequenced. Each clone was found to possess a series of repeated sequences of 41, 30 and 163 bases respectively. In pAnql and pAnml4 the repeats were in direct tandem array, whilst in pAna1 the repetitive sequence was found to be interspersed by 15-17 variable bases. A comparison of a number of copies of each of the repetitive sequences within the three clones enabled the definition of the consensus sequence for each repetitive element. Based on these consensus sequences, three oligonucleotides of 21, 23 and 26 bases were derived from pAna1, pAnq1 and pAnml4 respectively. When tested as probes against DNA dot-blots and squash-blots of mosquito specimens, each oligonucleotide retained the same species-specificity as the original clones from which they were derived. The radioactively labelled oligonucleotides were able to detect as little as 5 ng of target genomic DNA in an overnight autoradiographic exposure. The synthetic DNA probes will form the basis of a simplified system for the field identification of Anopheles gambiae sibling species specimens.

引用

页码：455 / 463

页数：9

共 23 条

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