PEAK IDENTIFICATION OF THE CONJUGATED BILE-ACIDS IN REVERSED-PHASE LIQUID-CHROMATOGRAPHY BY USING THE CONJUGATION SELECTIVITY

It has been found for the first time that the selectivity between five glycine and taurine conjugated bile acids remains constant despite a considerable variation in retention values for each pair of the compounds in reversed-phase liquid chromatography (RPLC). The dependence of parameter S on log k'w in retention equation log k' = log k'w-S-phi results in parallel lines for glycine and taurine conjugates. The selectivity between glycine and taurine conjugates has been utilized for the identification of the conjugated bile acids in RPLC.