MUTATION PROXIMAL TO THE TRANSFER-RNA BINDING REGION OF THE NICOTIANA PLASTID 16S RIBOSOMAL-RNA CONFERS RESISTANCE TO SPECTINOMYCIN

被引:67
作者
SVAB, Z
MALIGA, P
机构
[1] Waksman Institute, Rutgers, The State University of New Jersey, Piscataway, 08855-0759, NJ
来源
MOLECULAR & GENERAL GENETICS | 1991年 / 228卷 / 1-2期
关键词
NICOTIANA-TABACUM; PLASTID MUTANTS; 16S RIBOSOMAL RNA; SPECTINOMYCIN RESISTANCE; STREPTOMYCIN RESISTANCE;
D O I
10.1007/BF00282483
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nicotiana tabacum lines carrying maternally inherited resistance to spectinomycin were obtained by selection for green callus in cultures bleached by spectinomycin. Two levels of resistance was found. SPC1 and SPC2 seedlings are resistant to high levels (500-mu-g/ml), SPC23 seedlings are resistant to low levels (50-mu-g/ml) of spectinomycin. Lines SPC2 and SPC23 are derivatives of the SR1 streptomycin-resistant plastome mutant. Spectinomycin resistance is due to mutations in the plastid 16S ribosomal RNA: SPC1, an A to C change at position 1138; SPC2, a C to U change at position 1139; SPC23, a G to A change at position 1333. Mutations similar to those in the SPC1 and SPC2 lines have been previously described, and disrupt a conserved 16S ribosomal RNA stem structure. The mutation in the SPC23 line is the first reported case of a mutation close to the region of the 16S rRNA involved in the formation of the initiation complex. The new mutants provide markers for selecting plastid transformants.
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页码:316 / 319
页数:4
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