THE DIFFERENTIAL-EFFECTS OF POLYMORPHONUCLEAR LEUKOCYTE SECRETION ON HUMAN NATURAL-KILLER-CELL ACTIVITY

被引：4

作者：

LALA, A ^{[1
]}

LINDEMANN, RA ^{[1
]}

MIYASAKI, KT ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,SCH DENT,ORAL BIOL SECT,13-089 CHS,LOS ANGELES,CA 90024

来源：

ORAL MICROBIOLOGY AND IMMUNOLOGY | 1992年 / 7卷 / 02期

关键词：

POLYMORPHONUCLEAR LEUKOCYTE; NK CELL; LAK CELL; CYTOTOXICITY; SECRETION;

D O I：

10.1111/j.1399-302X.1992.tb00515.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

We studied the differential effects of polymorphonuclear leukocyte (PMN) secretion on human natural killer cell (NK) and lymphokine-activated killer cell (LAK) activity. Supernatant fluids from PMN stimulated by serum-opsonized zymosan (SOZ), n-formylmethionylleucylphenylalanine (FMLP) and interleukin-8 (IL-8) were incubated with peripheral blood lymphocytes (PBL) for 1 d and 4 d. Supernates from unstimulated PMN and PMN induced by FMLP and IL-8 decreased NK and LAK cytotoxicity in a dose-dependent fashion against K562 and M14 targets, respectively. Only the suppression caused by supernates from unstimulated PMN was ablated by incubation of PMN with indomethacin. Secretions from PMN stimulated by SOZ increased both NK and LAK cytotoxicity and induced PBL proliferation synergistically with IL-2. This enhancing factor was heat-labile, nondialyzable (MWCO 3500), and not blocked by anti-interferon-gamma. Anaerobic conditions did not influence the modulatory activity of PMN supernates, indicating that oxygen metabolites were not involved. We conclude that PMN release factors that modulate in vitro NK and LAK activities.

引用

页码：89 / 95

页数：7

共 42 条

[1]

Baggiolini M, Bretz U, Dewald B, Sub‐cellular localization of granulocyte enzymes, Neutral proteases of human polymorphonuclear leukocytes, pp. 3-17, (1978)

[2]

Bainton DF, Sequential degranulation of the two types of polymorphonuclear leukocyte granules during phagocytosis of microorganisms, J Cell Biol, 58, pp. 249-262, (1973)

[3]

Balkwill FR, Burke F, The cytokine network, Immunol Today, 10, pp. 299-304, (1989)

[4]

Berger L, Broida D, (1980)

[5]

Bradford MM, A rapid sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein‐dye binding, Anal Biochem, 72, pp. 248-254, (1976)

[6]

Chance B, Maehly AC, Catalases and peroxidases, Methods Enzymol, 2, pp. 764-775, (1955)

[7]

Corrigan JJ, Sieber OF, Ratajczak H, Bennet BB, Modification of human neutrophil response to endotoxin with polymyxin B sulfate, J Infect Dis, 130, pp. 384-387, (1974)

[8]

Decker LA, Lysozyme (muramidase). Worthington enzyme manual, pp. 185-188, (1977)

[9]

El-Hag A, Clark RA, Immunosuppression by activated human neutrophils. Dependence on the myeloperoxide system, J Immunol, 139, pp. 2406-2413, (1987)

[10]

Garcia-Penarubia P, Koster FT, Kelley RO, McDowell TD, Bankhurst AD, Antibacterial activity of natural killer cells, Journal of Experimental Medicine, 169, pp. 99-113, (1989)

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