DNA methylation changes during mouse spermatogenesis

被引：43

作者：

del Mazo, Jesus ^{[1
]}

Prantera, Giorgio ^{[2
]}

Torres, Miguel ^{[1
]}

Ferraro, Marina ^{[3
]}

机构：

[1] Ctr Invest Biol CSIC, Madrid 28006, Spain

[2] Univ Tuscia, Dept Agrobiol & Agrochim, Viterbo, Italy

[3] Univ Rome La Sapienza, Dept Genet & Biol Mol, Rome, Italy

来源：

CHROMOSOME RESEARCH | 1994年 / 2卷 / 02期

关键词：

gametogenesis; imprinting; in situ nick-translation; methylation; mouse; Mus musculus; spermatogenesis;

D O I：

10.1007/BF01553493

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genomic imprinting in mammals is thought to be mediated by differences in the methylation level of cytosine residues in the genome. These differences in DNA methylation are thought to be generated during the development of the germ line. To characterize the profile of global methylation of the mouse genome during male gametogenesis, we have quantified the relative level of methylation in individual cells during meiosis and spermatogenesis. A decrease in the level of DNA methylation is observed from meiotic cells to elongated spermatids. The erasure of the somatic pattern of methylation during spermatogenesis suggests the existence of a subsequent mechanism generating the parental specific methylation patterns leading to genomic imprinting of specific alleles.

引用

页码：147 / 152

页数：6

共 27 条

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