DEVELOPMENT OF CELL MARKERS IN SUBRETINAL RABBIT RETINAL TRANSPLANTS

被引：23

作者：

BERGSTROM, A

EHINGER, B

WILKE, K

ZUCKER, CL

ADOLPH, AR

SZEL, A

机构：

[1] LUND UNIV, DEPT OPHTHALMOL, LUND, SWEDEN

[2] SCHEPENS EYE RES INST, BOSTON, MA USA

[3] HARVARD UNIV, SCH MED, BOSTON, MA 02115 USA

[4] GOTHENBURG UNIV, DEPT ZOOL, S-40031 GOTHENBURG, SWEDEN

来源：

EXPERIMENTAL EYE RESEARCH | 1994年 / 58卷 / 03期

关键词：

RETINA; RETINAL TRANSPLANTS; DEVELOPMENT; IMMUNOHISTOCHEMISTRY; RHODOPSIN; CONE-SPECIFIC ANTIGENS; VISUAL PIGMENTS; PROTEIN KINASE C; GABA; HPC-1; INTERMEDIATE FILAMENTS;

D O I：

10.1006/exer.1994.1021

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Retinas from embryonic rabbits at day E15 were transplanted to the subretinal space in adult rabbits. After survival times between 7 and 193 days, the rabbits were killed, and the transplants were processed for immunohistochemistry. The results show that subretinal transplants from embryonic rabbit retinas develop many, if not all, retinal neuronal types. The cells show approximately normal morphology and express a variety of cell-type-specific markers: photoreceptor cells express visual pigment proteins as identified by antibodies against rhodopsin (R2-15), color-specific cone pigments (COS-1, OS-2) and the cone specific antigen 50-1B11, rod bipolar cells express PKC, horizontal cells HPC-1 antigen and neurofilament 160 kDa, amacrine cells HPC-1 antigen, GABA and neurofilament 160 kDa, and glial cells express vimentin and glial fibrillary acidic protein. The high degree of rosette formation seen in many young grafts, diminishes with time; many transplant cells disappear, and the remaining cells present a less prominent formation of rosettes. © 1994 Academic Press. All rights reserved.

引用

页码：301 / 313

页数：13

共 43 条

[1] CROSS-SPECIES GRAFTING OF EMBRYONIC MOUSE AND GRAFTING OF OLDER POSTNATAL RAT RETINAS INTO THE LESIONED ADULT-RAT EYE - THE IMPORTANCE OF CYCLOSPORIN-A FOR SURVIVAL [J].