DETECTION AND PARTIAL SEQUENCING OF HEPATITIS-C VIRUS-RNA IN THE LIVER

被引:16
作者
HOSODA, K [1 ]
YOKOSUKA, O [1 ]
OMATA, M [1 ]
KATO, N [1 ]
OHTO, M [1 ]
机构
[1] CHIBA UNIV,SCH MED,DEPT MED 1,1-8-1 INOHANA,CHIBA 280,JAPAN
关键词
D O I
10.1016/0016-5085(91)90537-U
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
To detect hepatitis C virus RNA, total RNA was extracted from liver tissue, reverse transcribed to complementary DNA, and amplified by polymerase chain reaction. The reaction products were analyzed by ethidium bromide staining in acrylamide gel and hybridization with a radiolabeled probe. Hepatitis C virus RNA was thereby detected in 17 of 27 (63%) liver tissue specimens obtained from patients with non-A, non-B chronic liver diseases. Of these 27 patients, viral RNA was detected in 12 of 17 (71%) liver tissues from anti-hepatitis C virus-positive patients and in 5 of 10 (50%) liver tissues from anti-hepatitis C virus-negative patients. Direct sequencing of amplified complementary DNA (35 nucleotides) of the 17 RNA-positive samples showed only 66% to 77% homology to the reported hepatitis C virus complementary DNA sequence. These results indicate that the majority of anti-hepatitis C virus-positive patients are currently infected with hepatitis C virus, and some of the anti-hepatitis C virus-negative patients with non-A, non-B hepatitis are harboring hepatitis C virus in the liver. Detection of hepatitis C virus RNA appears to provide a useful indicator in the study of hepatitis C virus infection. © 1991.
引用
收藏
页码:766 / 771
页数:6
相关论文
共 12 条
[1]   DETECTION OF ANTIBODY TO HEPATITIS-C VIRUS IN PROSPECTIVELY FOLLOWED TRANSFUSION RECIPIENTS WITH ACUTE AND CHRONIC NON-A-HEPATITIS, NON-B-HEPATITIS [J].
ALTER, HJ ;
PURCELL, RH ;
SHIH, JW ;
MELPOLDER, JC ;
HOUGHTON, M ;
CHOO, QL ;
KUO, G .
NEW ENGLAND JOURNAL OF MEDICINE, 1989, 321 (22) :1494-1500
[2]   ISOLATION OF A CDNA CLONE DERIVED FROM A BLOOD-BORNE NON-A, NON-B VIRAL-HEPATITIS GENOME [J].
CHOO, QL ;
KUO, G ;
WEINER, AJ ;
OVERBY, LR ;
BRADLEY, DW ;
HOUGHTON, M .
SCIENCE, 1989, 244 (4902) :359-362
[3]  
HOUGHTON M, 1989, INT APPLICATION PUBL, P1
[4]   A CDNA FRAGMENT OF HEPATITIS-C VIRUS ISOLATED FROM AN IMPLICATED DONOR OF POST-TRANSFUSION NON-A, NON-B HEPATITIS IN JAPAN [J].
KUBO, Y ;
TAKEUCHI, K ;
BOONMAR, S ;
KATAYAMA, T ;
CHOO, QL ;
KUO, G ;
WEINER, AJ ;
BRADLEY, DW ;
HOUGHTON, M ;
SAITO, I ;
MIYAMURA, T .
NUCLEIC ACIDS RESEARCH, 1989, 17 (24) :10367-10372
[5]   AN ASSAY FOR CIRCULATING ANTIBODIES TO A MAJOR ETIOLOGIC VIRUS OF HUMAN NON-A, NON-B-HEPATITIS [J].
KUO, G ;
CHOO, QL ;
ALTER, HJ ;
GITNICK, GL ;
REDEKER, AG ;
PURCELL, RH ;
MIYAMURA, T ;
DIENSTAG, JL ;
ALTER, MJ ;
STEVENS, CE ;
TEGTMEIER, GE ;
BONINO, F ;
COLOMBO, M ;
LEE, WS ;
KUO, C ;
BERGER, K ;
SHUSTER, JR ;
OVERBY, LR ;
BRADLEY, DW ;
HOUGHTON, M .
SCIENCE, 1989, 244 (4902) :362-364
[6]   HISTOLOGICAL-CHANGES OF THE LIVER BY TREATMENT OF CHRONIC NON-A, NON-B HEPATITIS WITH RECOMBINANT LEUKOCYTE INTERFERON-ALPHA - COMPARISON WITH HISTOLOGICAL-CHANGES IN CHRONIC HEPATITIS-B [J].
OMATA, M ;
ITO, Y ;
YOKOSUKA, O ;
IMAZEKI, F ;
UCHIUMI, K ;
TAKANO, S ;
HOSODA, K ;
OHTO, M .
DIGESTIVE DISEASES AND SCIENCES, 1989, 34 (03) :330-337
[7]   PRIMER-DIRECTED ENZYMATIC AMPLIFICATION OF DNA WITH A THERMOSTABLE DNA-POLYMERASE [J].
SAIKI, RK ;
GELFAND, DH ;
STOFFEL, S ;
SCHARF, SJ ;
HIGUCHI, R ;
HORN, GT ;
MULLIS, KB ;
ERLICH, HA .
SCIENCE, 1988, 239 (4839) :487-491
[8]   DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].
SANGER, F ;
NICKLEN, S ;
COULSON, AR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467
[9]   APPEARANCE OF VIRAL-RNA TRANSCRIPTS IN THE EARLY STAGE OF DUCK HEPATITIS-B VIRUS-INFECTION [J].
TAGAWA, M ;
OMATA, M ;
OKUDA, K .
VIROLOGY, 1986, 152 (02) :477-482
[10]  
WEINER AJ, 1990, LANCET, P1