INDUCTION OF ANTIVIRAL IMMUNE-RESPONSES BY IMMUNIZATION WITH RECOMBINANT-DNA ENCODED AVIAN CORONAVIRUS NUCLEOCAPSID PROTEIN

被引：38

作者：

BOOTS, AMH

BENAISSATROUW, BJ

HESSELINK, W

RIJKE, E

SCHRIER, C

HENSEN, EJ

机构：

[1] UNIV UTRECHT,FAC VET MED,INST INFECT DIS & IMMUNOL,DEPT IMMUNOL,POB 80165,3508 TD UTRECHT,NETHERLANDS

[2] EIJKMAN WINKLER LAB,EXPTL VIROL SECT,UTRECHT,NETHERLANDS

[3] INTERVET INT BV,5830 AA BOXMEER,NETHERLANDS

来源：

VACCINE | 1992年 / 10卷 / 02期

关键词：

INFECTIOUS BRONCHITIS VIRUS; MICE; CHICKENS; NUCLEOCAPSID; RECOMBINANT DNA;

D O I：

10.1016/0264-410X(92)90028-I

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Immune responses to the infectious bronchitis virus (IBV) nucleocapsid protein were studied using a recombinant-DNA expression product. In mice, a lymphocyte proliferative response and a delayed-type hypersensitivity reaction to IBV were induced upon immunization with this nucleocapsid protein. Next, we studied the role of the expressed nucleocapsid protein in induction of a protective immune response to IBV in chickens. Chickens were primed with nucleocapsid protein and subsequently boosted with inactivated IBV, strain M41. Proliferative responses of blood mononuclear cells corresponded with increased mean haemagglutination inhibition and virus neutralization titres. Finally, an increased tracheal protection against challenge with live IBV was observed. These results indicate that infectious bronchitis virus nucleocapsid protein is a relevant target for immune recognition in both the mouse and the chicken.

引用

页码：119 / 124

页数：6

共 38 条

[1] A STANDARD TECHNIQUE FOR HEMAGGLUTINATION INHIBITION TESTS FOR ANTIBODIES TO AVIAN INFECTIOUS-BRONCHITIS VIRUS [J].