AUTOMATED IMMUNOCYTOCHEMISTRY FOR CELLULAR MARKERS ON CELL SMEARS AND CYTOSPINS

被引：16

作者：

COWAN, DW

NOVOTNY, DB

MAYGARDEN, SJ

PRYZWANSKY, KB

机构：

[1] University of North Carolina, Department of Pathology, UNC Hospital, Chapel Hill, NC

来源：

JOURNAL OF HISTOTECHNOLOGY | 1992年 / 15卷 / 04期

关键词：

AUTOMATED IMMUNOHISTOCHEMISTRY; CYTOSPINS; FINE NEEDLE ASPIRATES;

D O I：

10.1179/his.1992.15.4.283

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A rapid, automated immunocytochemical staining method for the Instrumentation Laboratory Code-On stainer was developed to detect cellular markers in cell smears and cytospins prepared from fine needle aspirates of solid tumors and from body fluids collected by brushing, scraping, or washing. The acetone fixed cell smears and cytospins were stained with monoclonal and polyclonal commercially available antibodies and the Vectastain Elite Mouse and Rabbit kits. This system produced reproducible high quality, specific staining of lymphocyte, macrophage, epithelia, and prostatic markers with substantial time and cost savings over manual processing.

引用

页码：283 / 288

页数：6

共 6 条

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