PHOTOAFFINITY-LABELING OF THE NMDA RECEPTOR

被引：6

作者：

BENKE, D

MARTI, T

HECKENDORN, R

REHM, H

KUNZI, R

ALLGEIER, H

ANGST, C

MOHLER, H

机构：

[1] UNIV ZURICH,INST PHARMACOL,GLORIASTR 32,CH-8006 ZURICH,SWITZERLAND

[2] CIBA GEIGY LTD,RES DEPT,DIV PHARMACEUT,BASEL,SWITZERLAND

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION | 1993年 / 246卷 / 02期

关键词：

EXCITATORY AMINO ACID RECEPTORS; NMDA RECEPTOR ANTAGONIST; PHOTOAFFINITY LIGAND;

D O I：

10.1016/0922-4106(93)90096-R

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The structure of NMDA receptors in situ has been probed with the novel photoaffinity ligand I-125-CGP 55802A. By covalently linking the radioactive high-affinity photolabel to NMDA receptors in bovine brain we have identified a protein of 175 kDa associated with the binding site for NMDA receptor agonists and competitive antagonists. Based on its molecular size the photolabeled protein is likely to correspond to the NR2A and/or NR2B subunit. The photoaffinity ligand will permit the assessment of regulatory changes in NMDA receptor subunit expression.

引用

页码：179 / 180

页数：2

共 8 条

[1] EXCITATORY AMINO-ACID RECEPTORS AND SYNAPTIC PLASTICITY
COLLINGRIDGE, GL
SINGER, W
[J]. TRENDS IN PHARMACOLOGICAL SCIENCES, 1990, 11 (07) : 290 - 296
[2] CGP-37849 AND CGP-39551 - NOVEL AND POTENT COMPETITIVE N-METHYL-D-ASPARTATE RECEPTOR ANTAGONISTS WITH ORAL ACTIVITY
FAGG, GE
OLPE, HR
POZZA, MF
BAUD, J
STEINMANN, M
SCHMUTZ, M
PORTET, C
BAUMANN, P
THEDINGA, K
BITTIGER, H
ALLGEIER, H
HECKENDORN, R
ANGST, C
BRUNDISH, D
DINGWALL, JG
[J]. BRITISH JOURNAL OF PHARMACOLOGY, 1990, 99 (04) : 791 - 797
[3] ISHII T, 1993, J BIOL CHEM, V268, P2836
[4] MODULATION OF NEURONAL MIGRATION BY NMDA RECEPTORS
KOMURO, H
RAKIC, P
[J]. SCIENCE, 1993, 260 (5104) : 95 - 97
[5] MOLECULAR DIVERSITY OF THE NMDA RECEPTOR CHANNEL
KUTSUWADA, T
KASHIWABUCHI, N
MORI, H
SAKIMURA, K
KUSHIYA, E
ARAKI, K
MEGURO, H
MASAKI, H
KUMANISHI, T
ARAKAWA, M
MISHINA, M
[J]. NATURE, 1992, 358 (6381) : 36 - 41
[6] EXCITATORY AMINO-ACID NEUROTOXICITY AND NEURODEGENERATIVE DISEASE
MELDRUM, B
GARTHWAITE, J
[J]. TRENDS IN PHARMACOLOGICAL SCIENCES, 1990, 11 (09) : 379 - 387
[7] HETEROMERIC NMDA RECEPTORS - MOLECULAR AND FUNCTIONAL DISTINCTION OF SUBTYPES
MONYER, H
SPRENGEL, R
SCHOEPFER, R
HERB, A
HIGUCHI, M
LOMELI, H
BURNASHEV, N
SAKMANN, B
SEEBURG, PH
[J]. SCIENCE, 1992, 256 (5060) : 1217 - 1221
[8] MOLECULAR-CLONING AND CHARACTERIZATION OF THE RAT NMDA RECEPTOR
MORIYOSHI, K
MASU, M
ISHII, T
SHIGEMOTO, R
MIZUNO, N
NAKANISHI, S
[J]. NATURE, 1991, 354 (6348) : 31 - 37

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