N-3 FATTY-ACIDS STIMULATE INTRACELLULAR DEGRADATION OF APOPROTEIN-B IN RAT HEPATOCYTES

被引:124
作者
WANG, HX [1 ]
CHEN, XL [1 ]
FISHER, EA [1 ]
机构
[1] MED COLL PENN,DEPT BIOCHEM,2900 QUEEN LANE,PHILADELPHIA,PA 19129
关键词
APOPROTEIN B48; APOPROTEIN B100; APOPROTEIN-B DEGRADATION; IMMUNOPRECIPITATION; N-3 FATTY ACIDS;
D O I
10.1172/JCI116340
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
When rat hepatocytes were incubated with albumin complexed to the n-3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), rather than to oleic acid (OA), the secretion of newly synthesized apoprotein B100 (apoB100) or B48 (apoB48) was reduced, despite stimulation of cellular triglyceride synthesis by all three fatty acids. When pulse-chase studies of apoB synthesis and secretion were performed in the presence of OA, EPA, or DHA, there were no significant changes in the initial synthetic rates of either apoB species. However, during the chase period, the total recovery of labeled apoB100 and apoB48 from the cell and medium was less in the n-3 fatty acid groups, so that by 150 min, approximately half as much labeled apoB was recovered as in the OA group. Overall, the decreased accumulation in medium of labeled apoB in the presence of EPA and DHA could be quantitatively accounted for by increased degradation of intracellular apoB. Thus, in the primary hepatocyte, apoB degradation is not constitutive, but can be regulated by n-3 fatty acids.
引用
收藏
页码:1380 / 1389
页数:10
相关论文
共 45 条
[1]   POSSIBLE ROLE OF THE GOLGI-APPARATUS IN THE ASSEMBLY OF VERY LOW-DENSITY-LIPOPROTEIN [J].
BAMBERGER, MJ ;
LANE, MD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (07) :2390-2394
[2]   MACROPHAGES FROM NEPHROTIC RATS REGULATE APOLIPOPROTEIN-E BIOSYNTHESIS AND CHOLESTEROL CONTENT INDEPENDENTLY [J].
BASS, J ;
FISHER, EA ;
PRACK, MM ;
WILLIAMS, DL ;
MARSH, JB .
JOURNAL OF CLINICAL INVESTIGATION, 1991, 87 (02) :470-475
[3]  
BORCHARDT RA, 1987, J BIOL CHEM, V262, P16394
[4]  
BOREN J, 1990, J BIOL CHEM, V265, P10556
[5]  
BOSTROM K, 1986, J BIOL CHEM, V261, P3800
[6]   NEW VARIATION ON THE TRANSLOCATION OF PROTEINS DURING EARLY BIOGENESIS OF APOLIPOPROTEIN-B [J].
CHUCK, SL ;
YAO, ZM ;
BLACKHART, BD ;
MCCARTHY, BJ ;
LINGAPPA, VR .
NATURE, 1990, 346 (6282) :382-385
[7]  
CIANFLONE KM, 1990, J LIPID RES, V31, P2045
[8]  
DAVIDSON NO, 1988, J BIOL CHEM, V263, P13482
[9]  
DAVIS RA, 1982, J BIOL CHEM, V257, P908
[10]  
DAVIS RA, 1989, J LIPID RES, V30, P1185