A SIMPLE AND INEXPENSIVE DEVICE FOR FREEZE-SUBSTITUTION AT 183-K -90-C

被引：6

作者：

HESS, MW ^{[1
]}

GLASER, A ^{[1
]}

机构：

[1] UNIV VIENNA,BOT GARDEN,A-1030 VIENNA,AUSTRIA

来源：

BIOTECHNIC & HISTOCHEMISTRY | 1993年 / 68卷 / 04期

关键词：

FREEZE SUBSTITUTION; ULTRASTRUCTURE; POLLEN;

D O I：

10.3109/10520299309104700

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The freeze substitution device described here consists of commonly available materials, is easy to construct and to handle and provides maximal working safety. An adequate mass of copper and/or brass, serving as holder for 16 cryovials, is chilled to liquid nitrogen temperature. The vial holder is placed in a precooled thermos flask filled with ethanol and stored in an ultrafreezer at 193 K/-90 C. This ensures that the vial holder maintains temperatures of about 183 K/-90 C for more than 12 hr to minimize the risk of ice recrystallization.

引用

页码：211 / 214

页数：4