IN-VITRO BINDING OF THE 2-FINGER REPRESSOR CREA TO SEVERAL CONSENSUS AND NONCONSENSUS SITES AT THE IPNA UPSTREAM REGION IS CONTEXT-DEPENDENT

被引：50

作者：

ESPESO, EA ^{[1
]}

PENALVA, MA ^{[1
]}

机构：

[1] CSIC,CTR INVEST BIOL,E-28008 MADRID,SPAIN

来源：

FEBS LETTERS | 1994年 / 342卷 / 01期

关键词：

TRANSCRIPTION FACTOR; DNA BINDING; ZINC FINGER; ASCOMYCETES; CARBON CATABOLITE REPRESSION;

D O I：

10.1016/0014-5793(94)80581-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The two zinc-fingers of the Aspergillus nidulans repressor CreA recognize the consensus hexanucleotide 5'-SYGGRG-3'. We have determined all the CreA binding sites in a similar to 2 kb region upstream the ipnA gene. Our analysis shows that (i) CreA binds to certain consensus sites in a context-dependent manner; (ii) five non-consensus 6-bp sequences are also recognized by CreA; this non-canonical binding correlates with the presence of a second, neighbouring CreA binding site, suggesting that recognition of two linked sites stabilizes CreA binding. Our results suggest that the binding possibilities of CreA might be more complex than originally envisaged.

引用

页码：43 / 48

页数：6

共 19 条

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