ELISA METHODOLOGY FOR POLYSACCHARIDE ANTIGENS - PROTEIN COUPLING OF POLYSACCHARIDES FOR ADSORPTION TO PLASTIC TUBES

被引：188

作者：

GRAY, BM

机构：

[1] UNIV ALABAMA, SCH MED, DEPT PEDIAT, BIRMINGHAM, AL 35294 USA

[2] UNIV ALABAMA, SCH MED, DEPT MICROBIOL, BIRMINGHAM, AL 35294 USA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1979年 / 28卷 / 1-2期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0022-1759(79)90340-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described which permits the adaption of ELISA [enzyme-linked immunosorbent assay] techniques for measurement of [human] antibody against bacterial [streptococcal] polysaccharides [PS]. The PS antigen is covalently bound to poly-L-lysine using cyanuric chloride as the coupling agent. The poly-L-lysine then adsorbs to the walls of plastic tubes, immobilizing the PS coupled to the poly-L-lysine. The method is simple, rapid and utilizes small amounts of PS antigen.

引用

页码：187 / 192

页数：6