OPTIMIZED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC PROCEDURE FOR THE SEPARATION AND QUANTIFICATION OF THE MAIN FOLACINS AND SOME DERIVATIVES .1. CHROMATOGRAPHIC SYSTEM

被引：9

作者：

HAHN, A ^{[1
]}

STEIN, J ^{[1
]}

RUMP, U ^{[1
]}

REHNER, G ^{[1
]}

机构：

[1] UNIV GIESSEN,INST NUTR,WILHELMSTR 20,W-6300 GIESSEN,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 540卷 / 1-2期

关键词：

D O I：

10.1016/S0021-9673(01)88810-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An improved reversed-phase high-performance liquid chromatographic (HPLC) procedure for the rapid separation and sensitive fluorimetric quantification of the main folacins and some degradation products is presented. The system is the first to include two substances that can be used as internal standards. It allows the complete separation of all substances within 26 min. The fluorescence of reduced folates is measured directly after the column whereas other compounds are converted oxidatively to fluorescent products by post-column derivatization. Among several oxidants tested, potassium peroxodisulphate was found to be the most suitable.

引用

页码：207 / 215

页数：9

共 44 条

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