CLONING, CHARACTERIZATION, AND DNA-BASE SEQUENCE OF THE HIGH-LEVEL STREPTOMYCIN RESISTANCE GENE STRA1 OF HAEMOPHILUS-INFLUENZAE RD

被引：9

作者：

STUY, JH ^{[1
]}

WALTER, RB ^{[1
]}

机构：

[1] SW TEXAS STATE UNIV,DEPT BIOL,SAN MARCOS,TX 78666

来源：

JOURNAL OF BACTERIOLOGY | 1992年 / 174卷 / 17期

关键词：

D O I：

10.1128/JB.174.17.5604-5608.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The high-level streptomycin resistance strA1 gene of Haemophilus influenzae Rd was cloned in plasmid pAT4 as a 2.1-kbp EcoRI insert. It was later replaced in pAT4 by the wild-type strA+ gene. Plasmid pAT4 carrying the strA+ gene is highly unstable and renders chromosomally resistant recipients sensitive to streptomycin. The strA+ gene and the instability factor both reside on a 500-base HindIII-EcoRI subfragment. The two biological activities are also expressed in Escherichia coli. Both wild-type (strA+) and mutant (strA1) genes were sequenced. They show considerable nucleotide homology with the E. coli strA+ gene and its product.

引用

页码：5604 / 5608

页数：5

共 25 条

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